Apparatus for simultaneously acquiring multiple images of biological tissues and method thereof

Abstract

The invention relates to an apparatus for simultaneously acquiring multiple images of biological tissues and a method thereof. The apparatus comprises a light source mechanism and an optical imaging mechanism; the light source mechanism comprises a laser light source and a first incoherent visible light source and a second incoherent visible light source having significantly different molar absorption coefficients of oxyhemoglobin and deoxyhemoglobin; the optical imaging mechanism includes an imaging lens group, a filter switcher, and an optoelectronic imaging device disposed in sequence alonga light detecting path, and a visible light filter and a laser filter are embedded in a optical filter switching device to form a visible light filter channel and a laser filter channel. The apparatus can simultaneously acquire multiple images of biological tissues, pixel-level registration can be performed between different parameter images, so that the precise fusion of hemodynamic multi-parameter detection can be achieved, the full-frame image acquisition can be achieved, the reduction of the resolution of an imaging space is not required, and the spectra between different types of imagesdo not interfere with each other.

Description

Technical field [0001] The invention relates to a device and method capable of simultaneously acquiring multiple images of biological tissues. Background technique [0002] Multi-parameter detection of hemodynamics is of great significance to accurately and comprehensively evaluate the physiological state of organisms. Clinical monitoring and basic research in life sciences usually require simultaneous acquisition of biological tissue blood oxygen, blood volume, and blood flow. The traditional ultrasound Doppler blood flow measurement device requires probe contact and is a single-point measurement with low resolution, which is relatively limited in practical applications. [0003] In recent years, laser speckle blood flow imaging technology has become an optical imaging technology that can quickly obtain blood flow distribution and changes in biological tissues. It has the advantages of non-contact, no contrast agent, no scanning, and full-field imaging. The scientific applicatio...

AI Technical Summary

Problems solved by technology

However, this technical solution only uses some of the pixels (R and G) in the camera array for calculation, and the spatial resolution is reduced; in addition, the simultaneous irradiation of two light sources and the limited cut-off depth of the Bayer filter will make imaging difficult. There will be correlated interference between the two light sources

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