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A kind of immobilized quorum induction quenching bacteria composite gel bead and its preparation method and application

A quorum sensing and composite gel technology, applied to biochemical equipment and methods, immobilized on/in organic carriers, immobilized enzymes, etc., can solve the problem of reduced microbial activity, damage to the activity of target bacteria, and poor dispersion of materials Good and other problems, to achieve the effect of improving activity, delaying membrane fouling efficiency, and low price

Active Publication Date: 2021-06-18
HUNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Polyvinyl alcohol (PVA) is often used for the immobilization of microorganisms, but the polyvinyl alcohol pellets prepared by the traditional boric acid crosslinking method have the following problems: agglomeration and agglomeration will occur during the preparation process, making the dispersion of the material poor. Well, it affects the molding of the pellets; during the preparation process, it will damage the activity of the target bacteria, reduce the activity of the microorganisms, and affect the removal of the quorum sensing signal molecule AHL; It is easy to be damaged under certain conditions, which affects the stability of the pellets; the mass transfer resistance of the prepared pellets is large, which is not conducive to the improvement of degradation efficiency

Method used

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  • A kind of immobilized quorum induction quenching bacteria composite gel bead and its preparation method and application
  • A kind of immobilized quorum induction quenching bacteria composite gel bead and its preparation method and application

Examples

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Effect test

Embodiment 1

[0037] A kind of immobilized quorum sensing quenching bacteria composite gel beads, including gel beads composed of polyvinyl alcohol (PVA) and sodium alginate (SA), wherein the surface and interior of the gel beads are loaded with powder Activated charcoal (PAC) and quorum sensing quenching for sterilization.

[0038] In this example, the mass content of powdered activated carbon in the immobilized quorum induction quenching sterilizing composite gel pellet is 1%.

[0039] In this embodiment, the quorum sensing quenching bacteria is Rhodococcus sp.BH4.

[0040] In this embodiment, the immobilized quorum induction quenching bacteria composite gel beads form a layered network structure from dense to sparse from the outside to the inside, and the quorum induction quenching bacteria are attached to the layered network structure.

[0041] In this embodiment, the particle size of the immobilized quorum induction quenching bacteria composite gel beads is 3.5 mm.

[0042] A method ...

Embodiment 2

[0049] An immobilized quorum induction quenching bacteria composite gel bead is basically the same as the immobilized quorum induction quenching bacteria composite gel bead (A2) in Example 1, the only difference is: the immobilized population of Example 2 The mass content of the powdered activated carbon in the induction quenching sterilizing composite gel pellet is 0.5%.

[0050] A preparation method of the above-mentioned immobilized quorum induction quenching bacteria composite gel beads is basically the same as the preparation method of the immobilized quorum induction quenching bacteria composite gel beads (A2) in Example 1, the only difference is: implementation The consumption of powdered activated carbon is 0.5g in the preparation method of example 2.

[0051] The immobilized quorum induction quenching bacteria composite gel beads prepared in Example 2 are numbered A1.

Embodiment 3

[0053] An immobilized quorum induction quenching bacteria composite gel bead is basically the same as the immobilized quorum induction quenching bacteria composite gel bead (A2) in Example 1, the only difference is: the immobilized population of Example 3 The mass content of the powdered activated carbon in the induction quenching sterilizing composite gel pellet is 1.5%.

[0054] A preparation method of the above-mentioned immobilized quorum induction quenching bacteria composite gel beads is basically the same as the preparation method of the immobilized quorum induction quenching bacteria composite gel beads (A2) in Example 1, the only difference is: implementation The consumption of powdered activated carbon is 1.5g in the preparation method of example 3.

[0055] The immobilized quorum induction quenching bacteria composite gel pellet prepared in Example 3 is numbered A3.

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Abstract

The invention discloses an immobilized quorum induction quenching sterilized composite gel bead and its preparation method and application. The composite gel bead includes a gel bead composed of polyvinyl alcohol and sodium alginate, and is loaded on the surface and inside There are powdered activated carbon and quorum quenching for bacteria. The preparation method is as follows: mixing polyvinyl alcohol, sodium alginate, powdered activated carbon with water, mixing with quorum induction quenching sterilized bacteria suspension, and cross-linking the obtained mixed solution with boric acid and calcium chloride aqueous solution and sodium sulfate solution in sequence reaction to obtain composite gel beads. The composite gel beads of the present invention have the advantages of low cost, good mass transfer performance, high mechanical strength, good dispersibility, high biological activity, strong adsorption capacity, etc., and the preparation method thereof has the advantages of simple preparation process, convenient operation, high production efficiency, and Short cycle, high product yield and other advantages. The composite gel beads of the invention can effectively remove the quorum sensing signal molecule AHL and delay membrane fouling efficiency, and have good application prospects.

Description

technical field [0001] The invention belongs to the field of environmental functional materials and water treatment new technologies, and relates to an immobilized quorum induction quenching bacteria composite gel bead and its preparation method and application. Background technique [0002] Membrane biofouling in membrane bioreactor (Membrane bioreactor, MBR) is mainly caused by the deposition of suspended solids in the mixed liquid and the growth of biofilm on the membrane surface. In recent years, the use of quorum quenching (QQ) to control membrane biofouling has become a new technical method. Unicellular bacteria rely on some specific signaling molecules such as N-acyl homoserine lactone (N-acyl homoserine lactone, AHL) to communicate with each other and respond to changes in the surrounding environment, such as the production of extracellular polymers and soluble metabolites, biofilm formation, etc. Therefore, certain methods can be used to control the signaling mole...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N11/084C12N11/10B01D65/08
CPCB01D65/08C12N11/08C12N11/10
Inventor 曾茁桐唐碧肖嵘黄瑾辉顾岩岭史亚慧赖萃曾光明李华
Owner HUNAN UNIV
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