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Microbial agent for treating latrine pit and preparing method and application of microbial agent

A technology of microbial agent and dry toilet, which is applied in the field of microbial agent for dry toilet and its preparation, and achieves the effect of good application prospect

Inactive Publication Date: 2019-10-11
广州舒国生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The existing dry toilet treatment technologies have their own advantages and disadvantages. According to the survey, the method of combining microorganisms with instruments and equipment is more popular in the current dry toilet treatment

Method used

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  • Microbial agent for treating latrine pit and preparing method and application of microbial agent

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Experimental program
Comparison scheme
Effect test

preparation example Construction

[0020] 1. Preparation of Lactobacillus reuteri bacteria liquid

[0021] Pick a loop of Lactobacillus reuteri GIM 1.614 from the Lactobacillus slant medium and inoculate it into the liquid Lactobacillus seed medium, and culture it statically at 30-37°C for 12-48 hours until the strain is in the logarithmic During the growth period, the culture was stopped to obtain the first-class bacterial classification of Lactobacillus reuteri; 30-37°C, static airtight fermentation time 24-72 hours, to obtain Lactobacillus reuteri (Lactobacillus reuteri) GIM 1.614 bacterial liquid, said Lactobacillus reuteri slant medium contains 10.0g of peptone per liter, 10.0g of beef extract g, yeast extract 5.0g, diammonium hydrogen citrate [(NH 4 ) 2 HC 6 h 5 o 7 ] 2.0g, glucose (C 6 h 12 o 6 ·H 2 O) 20.0g, Tween 80 1.0mL, sodium acetate (CH 3 COONa·3H 2 O) 5.0g, dipotassium hydrogen phosphate (K 2 HPO 4 ·3H 2 O) 2.0g, magnesium sulfate (MgSO 4 ·7H 2 O) 0.58g, manganese sulfate (MnSO 4...

Embodiment 1

[0033] The above-mentioned Lactobacillus reuteri (Lactobacillus reuteri) GIM 1.614 bacterial liquid, Streptococcus thermophilus (Streptococcus thermophilus) CICC 201 bacterial liquid, Saccharomyces cerevisiae (Saccharomyces cerevisiae) ATCC 9763 Bacterial liquid, Trichoderma koningii (Trichoderma koningii) ACCC 30388 bacterial liquid and Bacillus coagulans (Bacillus coagulans) ATCC7050 bacterial liquid in bacterial content, then the adjusted Lactobacillus reuteri (Lactobacillus reuteri) GIM 1.614 bacterial liquid, Streptococcus thermophilus CICC 201 bacterial fluid, Saccharomyces cerevisiae ATCC 9763 bacterial fluid, Trichoderma koningii ACCC 30388 bacterial fluid and Bacillus coagulans ATCC 7050 bacterial fluid were mixed to form a composite bacteria liquid, so that the composite bacteria liquid contains Lactobacillus reuteri (Lactobacillus reuteri) GIM 1.6141.5 × 10 10 Individual / L, Streptococcus thermophilus CICC 201 1.5×10 12 Units / L, Saccharomyces cerevisiae ATCC 9763 2....

Embodiment 2

[0038] The above-mentioned Lactobacillus reuteri (Lactobacillus reuteri) GIM 1.614 bacterial liquid, Streptococcus thermophilus (Streptococcus thermophilus) CICC 201 bacterial liquid, Saccharomyces cerevisiae (Saccharomyces cerevisiae) ATCC 9763 Bacterial liquid, Trichoderma koningii (Trichoderma koningii) ACCC 30388 bacterial liquid and Bacillus coagulans (Bacillus coagulans) ATCC7050 bacterial liquid in bacterial content, then the adjusted Lactobacillus reuteri (Lactobacillus reuteri) GIM 1.614 bacterial liquid, Streptococcus thermophilus CICC 201 bacterial fluid, Saccharomyces cerevisiae ATCC 9763 bacterial fluid, Trichoderma koningii ACCC 30388 bacterial fluid and Bacillus coagulans ATCC 7050 bacterial fluid were mixed to form a composite bacteria liquid, so that the composite bacteria liquid contains Lactobacillus reuteri (Lactobacillus reuteri) GIM 1.6142.5 × 10 10 Individual / L, Streptococcus thermophilus CICC 201 3.5×10 12 Individuals / L, Saccharomyces cerevisiae ATCC 9...

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Abstract

The invention discloses a microbial agent for treating a latrine pit and a preparing method and application of the microbial agent. The microbial agent for treating the latrine pit is a compound microbial agent which is formed by mixing five strains in a certain proportion; after the microbial agent is mixed with the ground latrine pit according to a corresponding quantity, the latrine pit can bealmost degraded completely within 24 hours at the temperature of 70-90 DEG C and converted into usable microbial organic fertilizer. According to the microbial agent and the preparing method and application thereof, the scientific purposes of quick recycling, hazard-free treatment and quantity reduction of the latrine pit are achieved in situ, and the microbial agent has a great application prospect.

Description

Technical field: [0001] The invention belongs to the field of harmless treatment of dry toilet excrement, and in particular relates to a microbial agent for treating dry toilets, a preparation method and application thereof. Background technique: [0002] At present, there are six types of rural household toilets being promoted: dry toilet, three-compartment septic tank, double-urn funnel, three-connected biogas digester, excrement and urine diversion, double-pit alternate and sewer flushing. As a waterless or water-saving toilet, dry toilet integrates modern technology products such as industrial technology, information technology, and microbial technology. The feces in dry toilets have potential hazards of disease transmission. Improper handling will produce parasites and pathogenic bacteria, which will seriously endanger human health. In order to promote the toilet revolution more healthily and protect the health of the people, the implementation of harmless treatment of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/18C12N1/14C05F15/00C05F17/00C12R1/225C12R1/46C12R1/865C12R1/885C12R1/07
CPCC05F3/00C05F17/00C12N1/14C12N1/18C12N1/20C05F11/08Y02W30/40
Inventor 张国立罗旭张国华张国芝
Owner 广州舒国生物科技有限公司
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