Tissue culture method for one-step planting of clustered seedlings by tissue culture of Jingpinia

Abstract

The invention provides a tissue culture method for one-step seed formation by using Jingzhou pinellia ternate tissue-culture cluster seedlings. The method includes the following steps that 1, with-leaf petioles are taken as explants; 2, the explants are inoculated into a seed formation medium for culture to form bulbils and / or apical buds, and then the buds grow into seedlings after maturation, wherein the seed formation medium includes 1.3-1.7 mg / L of 1 / 2MS + IBA, 0.2-0.8 mg / L of NAA, 0.05-0.15 mg / L of TDZ, 3% of sucrose and 0.7% of agar. The with-leaf petioles of the Jingzhou pinellia ternate tissue-culture cluster seedlings serve as the explants, the special culture medium is adopted, formation of the bulbil and apical bud structures is facilitated, the bulbil and apical bud structures are similar to seed structures, the bulbils and apical buds can grow into the normal seedlings after maturation, the germination rate and the callus rate are high, no seedling exercising is required, seeds can be directly sowed, the survival rate reaches 100%, the quality of the seedlings is guaranteed, and scale production is achieved.

Description

technical field [0001] The invention relates to the technical field of asexual propagation of seedlings, in particular to a tissue culture method for cultivating bushy seedlings in one step by utilizing Jingpinia tissue culture. Background technique [0002] Pinellia belongs to the genus Pinellia of the family Araceae, and its dried tubers are used as medicine. It is an important traditional Chinese medicinal material. The tubers of Pinellia contain chemical components such as volatile oil, nicotine, glutamic acid, and sitosterol. It has the functions of lowering adverse flow and antiemetic, drying dampness and resolving phlegm, eliminating mass and resolving stagnation, etc. The ancient "Shenlong Materia Medica" called Pinellia Shoutian and Shuiyu. It is a perennial herb, mostly excavated in summer and autumn. Its tubers are spherical or oblate, and it likes sandy soil. The exact bulk of traditional Chinese medicinal materials. The original Jingzhou Youjiangling and Qianj...

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Problems solved by technology

[0004] Comparative Document 1: The document "Research on the Mechanism of Somatic Embryogenesis Induced by Lingfasu" records that Lingfasu has a certain effect on the induction of somatic embryos, but its healing rate and germination rate are low, and the price of Lingfasu is relatively low. High, higher overall cost

[0005] Comparative document 2: The application number is "CN102228005A", and the name is "Penellia tissue culture one-step seeding method". In the disclosed method, it takes 50 days for seedlings to grow, and it takes 30-40 days to form small tubers before transplanting. In the process of seedling formation, it is also necessary to add liquid medium, which requires a long cost and time, and the process of seedling formation is cumbersome and takes too long to meet the needs of the market.

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