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Method for culturing clostridium butyricum through mixed fermentation

A technology of clostridium butyricum and mixed culture, applied in the field of microbial culture, can solve the problems of no significant increase in biological yield and less research, and achieve the effects of great economic value, simple operation and improved biological activity

Inactive Publication Date: 2019-09-17
XIAMEN CANCO BIOTECH CO LTD
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  • Summary
  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are few studies on the mixed fermentation culture of Clostridium butyricum, and the biological yield obtained by the culture has not been significantly improved.

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0026] The mixed culture medium is calculated in 1L, including the following components: glucose 20g, peptone 30g, yeast powder 20g, K 2 HPO 4 0.5g, MgSO4 7H 2 O 0.5g, light CaCO 3 1g, MnSO 4 ·H 2 The distilled water of 00.02g and surplus, the pH value of the medium of described mixed culture is 7.2.

[0027] The medium used for the separate culture of Clostridium butyricum is a mixed culture medium, the composition of which is as above.

[0028] The medium used for the single culture of Bacillus subtilis is calculated in 1L: including 20g of glucose, 15g of peptone, 5g of sodium chloride, 0.5g of beef extract, 20g of agar and the rest of distilled water; pH value 6.8~7.2

[0029] The medium used for yeast culture alone is calculated in 1L: including 30g of sucrose, NaNO 3 3g,K 2 HPO 4 1g, KCL0.5g, MgSO 4 ·7H 2 O 0.5g, FeSO 40.01g, 15-20g of agar and the rest of distilled water, the pH value is 6.8-7.2.

[0030] The medium used for lactic acid bacteria culture alon...

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Abstract

The invention provides a method for culturing clostridium butyricum through mixed fermentation, and belongs to the technical field of microbial culture. The method includes the following step that single-bacterium culture solutions of clostridium butyricum, bacillus subtilis, yeast and lactic acid bacteria are mixed for mixed culture, wherein the temperature of mixed culture is 29 DEG C, the pH value of mixed culture is 6.4-6.8, the time for mixed culture is 16-20 hours, the rotating speed of mixed culture is 150-170 rpm, and the mixing ratio of clostridium butyricum to yeast to bacillus subtilis to lactic acid bacteria is 1:1:3:1. The method is simple to operate, the biological activity of cultured clostridium butyricum is remarkably improved, and the biological activity is greatly improved.

Description

technical field [0001] The invention belongs to the technical field of microorganism culture, and in particular relates to a method for culturing Clostridium butyricum by mixed fermentation. Background technique [0002] The rapid development of science and technology has significantly improved the living standards of human beings, and the increasingly prominent environmental protection and food safety issues have attracted more and more attention. The antibiotics that were favored by the aquaculture and feed industries earlier are gradually being replaced by greener and safer probiotics. As a member of the new probiotics family, Clostridium butyricum has a very broad market prospect for the aquaculture industry and other related industries. [0003] Clostridium butyricum is a non-resident, spore-forming probiotic that exists in the intestines of humans and animals, and is also widely distributed in leaves, soil, cheese, and natural yogurt. Inactivation), acid resistance (...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/16C12R1/145C12R1/125
CPCC12N1/16C12N1/20
Inventor 袁学锋姜宗然
Owner XIAMEN CANCO BIOTECH CO LTD
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