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Optical fiber measurement based three-dimensional heart chip and detection method thereof

A technology of optical fiber measurement and detection method, which is applied in the direction of measuring devices, using optical devices to transmit sensing components, converting sensor outputs, etc., can solve the problems of tediousness, inability to accurately capture myocardial movement, and large errors in small deflection detection. Application prospect, visual real-time detection effect

Active Publication Date: 2019-09-13
INST OF MEDICAL DEVICES (SUZHOU) SOUTHEAST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the process of preparing PDMS templates containing fixed polymer wires is complex and cumbersome, and the observation method is direct observation with a low-power microscope, so tiny myocardial movements cannot be accurately captured, and the algorithm for the contractile mechanical properties of myocardial bundles is based on microscopic image recognition Technology, the detection error for small deflection is large

Method used

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  • Optical fiber measurement based three-dimensional heart chip and detection method thereof
  • Optical fiber measurement based three-dimensional heart chip and detection method thereof
  • Optical fiber measurement based three-dimensional heart chip and detection method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1 3

[0037] Example 1 Three-dimensional heart chip is used for adrenaline detection

[0038] Prepare the mold of the elastic cantilever 1 by machining;

[0039] Cleaning the mold, pouring polydimethylsiloxane, peeling off after curing, and preparing a hollow elastic cantilever 1;

[0040] Perform aseptic treatment on the elastic cantilever 1, put it in a 96-well plate to fix the position, add the cell gel mixture 11, put it in the incubator for 1 hour, and add the medium 3 to culture after the gel solidifies;

[0041]After culturing for 3 days, add electrode 4, set the stimulation voltage, frequency, duration, etc. for electrical stimulation, and change the culture medium every other day to continue culturing for 3 weeks;

[0042] Three-dimensional myocardial tissue 2 overall contraction and relaxation;

[0043] Put the fiber optic probe into the hollow elastic cantilever 1 and fix it. Set the stimulation voltage, frequency, and duration to make the three-dimensional myocardial ...

Embodiment 2 3

[0044] Example 2 Three-dimensional heart chip is used for nifedipine detection

[0045] Prepare the mold of the elastic cantilever 1 by machining;

[0046] Cleaning the mold, pouring polydimethylsiloxane, peeling off after curing, and preparing a hollow elastic cantilever 1;

[0047] Perform aseptic treatment on the elastic cantilever 1, put it in a 96-well plate to fix the position, add the cell gel mixture 11, put it in the incubator for 1 hour, and add the medium 3 to culture after the gel solidifies;

[0048] After culturing for 3 days, add electrode 4, set the stimulation voltage, frequency, duration, etc. for electrical stimulation, and change the culture medium every other day to continue culturing for 3 weeks;

[0049] Three-dimensional myocardial tissue 2 overall contraction and relaxation;

[0050] Put the fiber optic probe into the hollow elastic cantilever 1 and fix it. Set the stimulation voltage, frequency, and duration to make the three-dimensional myocardial...

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Abstract

The invention discloses an optical fiber measurement based three-dimensional heart chip. According to the chip, elastic cantilevers serve as a support for three-dimensional myocardial tissue and can be continuously deformed and displaced in the contraction and relaxation processes of the three-dimensional myocardial tissue so as to be detected by optic fiber probes in the elastic cantilevers; under the action of contraction and relaxation behaviors of the three-dimensional myocardial tissue, the two cantilevers repeatedly get close and move far away, the distance between the two cantilevers ischanged and the characteristics of the beating frequency, the contraction force and the like of the myocardial tissue are reflected by the change frequency and the displacement amplitude; the changeof the elastic cantilevers in which optical fiber is wrapped is monitored by utilizing the characteristics of sensitivity, accuracy and the like of optical fiber measurement technology, thereby realizing the detection of important indexes such as the myocardial beating frequency, the contraction force and the like; and after a drug is added, the indexes such as the beating frequency, the contraction force and the like of the three-dimensional myocardial tissue are changed, drug evaluation and screening can be carried out by utilizing the data obtained by the optical fiber monitoring, the effect of the drug on myocardium can be observed in real time and a good visualization effect is provided.

Description

technical field [0001] The invention relates to a three-dimensional heart chip, in particular to a three-dimensional heart chip, a preparation method thereof, and an optical fiber measurement method for important parameters of the myocardium, belonging to the field of biological tissue engineering organ chips. Background technique [0002] The heart is one of the most important organs of the human body. Constructing functional heart substitutes is highly challenging due to the limited proliferative capacity of mature cardiomyocytes compared to other tissues. On the other hand, cardiotoxicity is one of the most common reasons for new drugs to be recalled during the human testing phase and even after the drug is marketed, accounting for one-third of the total number of drugs withdrawn from the market. extensive attention and attention. [0003] The use of two-dimensional cell static culture and animal models to predict human responses to new drugs has a high failure rate, ma...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/483G01D5/26
CPCG01D5/268G01N33/4833
Inventor 顾忠泽陈早早欧阳珺王菲孙晓玮葛健军张静
Owner INST OF MEDICAL DEVICES (SUZHOU) SOUTHEAST UNIV
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