Preparation method of chitosan/desoxyribonucleic acid modified electrode and method for electrochemically recognizing tryptophan enantiomers by using electrode

A technology of deoxyribonucleic acid and tryptophan enantiomers, which is applied in the electrochemical analysis and biological fields, can solve the problems of low sensitivity, complicated operation, and high analysis cost, and achieve the effect of cheap preparation method and improved recognition effect

Pending Publication Date: 2019-09-13
CHANGZHOU UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Some existing spectroscopic analysis methods have shortcomings such as low sensitivity, complicated operation, and high analysis cost, so it is very important to find an analytical method with good prospects

Method used

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  • Preparation method of chitosan/desoxyribonucleic acid modified electrode and method for electrochemically recognizing tryptophan enantiomers by using electrode
  • Preparation method of chitosan/desoxyribonucleic acid modified electrode and method for electrochemically recognizing tryptophan enantiomers by using electrode
  • Preparation method of chitosan/desoxyribonucleic acid modified electrode and method for electrochemically recognizing tryptophan enantiomers by using electrode

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Experimental program
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Effect test

Embodiment 1

[0021] Preparation of Chitosan / DNA Membrane for Scanning Electron Microscopy Characterization

[0022] a, take by weighing 50mg chitosan, dissolve in 50mL 1% acetic acid solution (volume ratio) and make 1mg / mL chitosan solution.

[0023] b. Weigh 50 mg of deoxyribonucleic acid (purchased from aladdin) and dissolve it in 50 mL of deionized water to prepare a 1 mg / mL deoxyribonucleic acid solution.

[0024] c. Wash the silicon wafer and dry it with nitrogen, place it in a chitosan solution of 1 mg / mL, and soak it for 15 minutes to obtain a chitosan-modified silicon wafer

[0025] d. Wash the chitosan-modified silicon wafer prepared in step c with deionized water and then immerse it in a deoxyribonucleic acid solution containing 1 mg / mL for 15 minutes to obtain a chitosan / deoxyribonucleic acid membrane.

Embodiment 2

[0027] Chitosan-modified electrodes, deoxyribonucleic acid-modified electrodes, and chitosan / deoxyribonucleic acid-modified glassy carbon electrodes were prepared for electrochemical recognition of tryptophan enantiomers.

[0028] Proceed as follows:

[0029] Preparation of chitosan-modified electrode: Prepare 4 mL of 1 mg / mL chitosan solution, immerse the glassy carbon electrode in the chitosan solution for 30 minutes, and obtain a chitosan-modified glassy carbon electrode.

[0030] Preparation of deoxyribonucleic acid modified electrode: prepare 1 mg / mL deoxyribonucleic acid solution, immerse the glassy carbon electrode in the chitosan solution for 30 minutes to obtain a deoxyribonucleic acid modified glassy carbon electrode.

[0031] Preparation of chitosan / deoxyribonucleic acid modified electrode for electrochemical chiral recognition of tryptophan enantiomers, the prepared chitosan modified electrode was washed with deionized water and then immersed in a deoxyribonucleic ...

Embodiment 3

[0039] The steps for preparing the chitosan / deoxyribonucleic acid modified electrode are as in Example 2. The prepared electrode was used as the working electrode, the Ag / AgCl electrode was used as the reference electrode, and the platinum wire electrode was used as the counter electrode. The three-electrode system was respectively immersed in 10 mL of PBS containing different mixing ratios of L-tryptophan and D-tryptophan. in solution. The DPV test was carried out in the potential window of 0.4-1V, and the graph of the change of current with the content of L-tryptophan was obtained. Such as Image 6 Shown: The current has a linear relationship with the L-tryptophan content.

[0040] Beneficial effects of the invention: the preparation method of the chitosan / deoxyribonucleic acid modified electrode is cheap, environmentally friendly and simple, and the modified electrode has a remarkable recognition effect on the enantiomer of tryptophan.

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Abstract

The invention relates to a preparation method of a chitosan / desoxyribonucleic acid modified electrode and a method for electrochemically recognizing tryptophan enantiomers by using the electrode. Thepreparation method comprises the following steps of firstly preparing a chitosan modified electrode; and placing the chitosan modified electrode in desoxyribonucleic acid solution for further modification so as to prepare the chitosan / desoxyribonucleic acid modified electrode which is used for electrochemically recognizing tryptophan enantiomers. The chitosan / desoxyribonucleic acid modified electrode has the effects of being simple in preparation process, environment-friendly and low in cost and having a remarkable recognition effect for the tryptophan enantiomers.

Description

technical field [0001] The invention relates to the preparation of a chitosan / deoxyribonucleic acid modified electrode and a method for electrochemically identifying tryptophan enantiomers, belonging to the fields of electrochemical analysis and biotechnology. Background technique [0002] Most amino acids in living systems are enantioselective. Chiral molecules with different configurations have significant differences in toxicity, biochemical activity, transport and metabolic mechanisms. Therefore, chiral recognition has always been a hotspot in chemistry and biology research. Some existing spectroscopic analysis methods have disadvantages such as low sensitivity, complicated operation, and high analysis cost, so it is very important to find an analytical method with good prospects. The electrochemical analysis method has the advantages of high sensitivity, fast response time, and easy operation, and can distinguish different amino acid enantiomers by adjusting the chira...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/327G01N27/30
CPCG01N27/308G01N27/3277
Inventor 孙一新何家辉张嵘盛扬徐德峰
Owner CHANGZHOU UNIV
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