Extraction method of estrogen in North China leopard excrement

Abstract

The invention belongs to the technical field of hormone extraction methods, and particularly relates to an extraction method of estrogen in North China leopard excrement. The method comprises the following steps of weighing 1 g of unfrozen North China leopard excrement in a centrifuge tube, and adding 10 ml of an ethanol solution with the mass concentration of 80%; placing in oscillating water bath at 70 DEG C for 15 minutes, cooling to 4 DEG C, putting into a centrifuge for centrifuging at 4000 r / min for 15 minutes, and taking supernate; adding 5 mL of an ethanol solution with the mass concentration of 80% into lower precipitate, carrying out vortex oscillation for 1 minute, putting into the centrifuge for centrifuging at 4000 r / min for 15 minutes, and merging the supernate; carrying outwater bath air drying on the merged supernate at the temperature of 60 DEG C; and adding PB for redissolving, and storing at -20 DEG C after redissolving is complete. The method is easy to operate andhigh in estrogen extraction rate, and has no damage to the North China leopard, and stress reaction of the North China leopard cannot be generated during an extraction process, so that a detection result is high in reliability.

Description

technical field [0001] The invention belongs to the technical field of hormone extraction methods, in particular to a method for extracting estrogen from the feces of North China leopards. Background technique [0002] The North China leopard is a subspecies of the leopard, belonging to the class Mammalia, order Carnivora, family Felidae, and large carnivores. In recent years, due to habitat destruction and other reasons, its number has decreased sharply, and it has been listed as a national first-level key protected animal; therefore, the reproduction of the North China leopard has attracted widespread attention from the industry. [0003] The estrogen level in the North China leopard has a very important effect on its reproduction, so it is necessary to carry out research on the estrogen in the North China leopard. At present, blood sample analysis is usually used to measure the estrogen in the North China leopard. Due to the difficulty in taking blood from the North Chin...

AI Technical Summary

Problems solved by technology

At present, blood sample analysis is usually used to measure the estrogen in the North China leopard. Due to the difficulty in taking blood from the North China leopard, it is necessary to capture, anesthetize or restrict the North China leopard. The damage is large, and the capture will cause stress to the animals, which will affect the estrogen level in the North China leopard. Therefore, the estrogen level measured by blood sample analysis may have a certain deviation from the true estrogen level in the North China leopard.

[0004] Feces are the natural metabolites of animals, which can truly reflect the changes in hormone levels in animals. Since collecting feces is relatively easy and does not harm animals, measuring the estrogen level in the feces of North China leopards will become a tool for studying its reproduction. An important direction, however, how to extract estrogen from the feces of the North China leopard has not been studied and reported