A Microlens Composite Microfluidic Channel for Improving Directional Luminescence of Fluorescent Substances

Abstract

The invention belongs to the field of fluorescence detection, and provides a microlens composite microfluidic channel structure for improving the directional light emission of fluorescent substances, including a glass substrate coated with a metal film with a thickness greater than or equal to 100nm, and above the metal film is a There are horizontally placed microfluidic tubes, and a solution containing fluorescent substances is arranged in the microfluidic tubes; the microfluidic tubes are formed by etching a hollow cylinder in the middle of a "racetrack" type dielectric column, and the "racetrack" type dielectric column The upper and lower ends of the body are arc-shaped, and the left and right ends are vertical. The invention can obtain a larger enhancement factor and can be widely used in the field of fluorescence detection.

Description

technical field [0001] The invention belongs to the field of fluorescence detection, and in particular relates to a microlens composite microfluidic channel for improving the directional luminescence of fluorescent substances, which can be applied to the field of directional luminescence when the distance between the fluorescent substance and the structure is greater than one wavelength. Background technique [0002] Fluorescence detection is a common detection method. Fluorescence detection has the characteristics of real-time, efficient, intuitive, and sensitive, and can be widely used in biomedical detection, environmental monitoring, chemical analysis and other fields. In fluorescence detection using fluorescent substances as fluorescent markers, the luminescent effect of fluorescent substances has a direct impact on detection sensitivity. In practice, the main factors that affect the intensity of fluorescent directional luminescence are: 1. The natural luminescence of ...

AI Technical Summary

Problems solved by technology

A common disadvantage of these enhancement methods is that when the enhancement methods of metal plasma structure and photonic crystal structure are used, the distance from the fluorescent substance to the structure must be below the sub-wavelength, and the luminescence of the fluorescent substance with a distance from the structure greater than one wavelength cannot be detected. effective enhancement

In short, the current method of using dielectric microlenses to enhance fluorescence is mainly to place microlenses on one side of the fluorescent material, and the fluorescence not within the aperture range of the microlenses cannot be collected. The directional enhancement effect of fluorescence is not good, and the enhancement factor is not high.

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