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A kind of method for cultivating seeds of quinoa tissue cultured seedlings under aseptic conditions

A breeding method, quinoa group technology, applied in horticultural methods, botanical equipment and methods, plant regeneration, etc., can solve the problems of difficult artificial detasseling and slow hybridization process of quinoa

Active Publication Date: 2022-03-04
YUNNAN AGRICULTURAL UNIVERSITY
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  • Abstract
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  • Claims
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Problems solved by technology

[0005] Quinoa is a floret plant (smaller and more flowers), and manual detasseling is difficult, so the hybridization process of quinoa is slow

Method used

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Embodiment

[0029] A method for cultivating seeds of quinoa tissue cultured seedlings under aseptic conditions, comprising the steps of:

[0030] S1, quinoa seed treatment:

[0031] Soak quinoa seeds in 75% alcohol for 30 seconds on a clean bench, then soak them in 0.1% mercuric chloride solution for 12 minutes, and then wash them with sterile water for 3-5 times;

[0032] S2, obtaining sterile seedlings

[0033] Inoculate the above-mentioned processed quinoa seeds on the medium of 1 / 2MS+0.5mg / L CCC to prepare sterile seedlings, germinate under no light conditions until the cotyledons grow out,

[0034] Among them: after the cotyledon grows out, the culture conditions are as follows: cultivate under the light, the temperature is 25±2°C, the relative humidity is 70-80%, the light intensity is 2500Lx, and the light time is 14h / d;

[0035] Obtain quinoa aseptic seedlings after 12 days;

[0036] S3, cutting quinoa seedlings to induce proliferation of adventitious buds

[0037] The above-m...

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Abstract

The invention discloses a method for cultivating seeds of quinoa tissue cultured seedlings under aseptic conditions, comprising the following steps: disinfecting quinoa seeds and inoculating them on a medium of 1 / 2MS+0.5mg / L CCC to prepare seedlings, and obtaining Aseptic quinoa seedlings; cut the aseptic quinoa seedlings for adventitious bud proliferation culture; transfer the multiplied adventitious buds to the medium of MS+1mg / L CCC to induce seeding. The present invention can realize the purpose of quinoa seeding every year under aseptic conditions, and at the same time, the purified seeds can be realized through the technical solution of the present invention. During the cultivation process, chlormequat (CCC) exists in the medium all the time, the time required from a seed to the aseptic seedling is consistent with the whole growth period of quinoa, about 100‑120d, and proliferates in adventitious buds Adventitious buds with infinitely multiplied stages are used to induce seed set, and sterile, pure-line seeds can be produced annually under tissue culture conditions for research.

Description

technical field [0001] The invention relates to the technical field of aseptic rapid propagation, in particular to a seeding cultivation method of quinoa tissue cultured seedlings under aseptic conditions. Background technique [0002] Quinoa, also known as quinoa, South American quinoa, etc., is an annual dicotyledonous plant of the family Amaranthaceae. Quinoa is a medicinal and edible crop, gluten-free, high in protein, amino acids, flavonoids, saponins and other elements, so it is called "golden grain" and is considered to be one of the crops that can ensure global food security in the future. [0003] Quinoa has been introduced and cultivated in more than 20 provinces and cities including Anhui, Gansu, Guizhou, Sichuan and Yunnan. However, the seed yield and biomass vary greatly in different regions, years and geographical conditions, mainly because the selection of quinoa varieties, variety matching, crop matching, weed control, harvesting and harvesting period manage...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/008A01H4/001
Inventor 董玉梅林春毛自朝刘正杰张玉敏袁加红
Owner YUNNAN AGRICULTURAL UNIVERSITY
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