Method of inoculating Ustilago scitaminea to tissue culture seedlings of sugarcane

Abstract

The invention discloses a method of inoculating Ustilago scitaminea to tissue culture seedlings of sugarcane. The method includes: with an injector, injecting a mixed spore suspension of '+' and '-' mated wild strains of Ustilago scitaminea and a mixed spore suspension of '+' and '-' mated mutant strains of Ustilago scitaminea respectively to inner parts, near growth spots, of the tissue culture seedlings of sugarcane, wherein the mixed spore suspension of the '+' and '-' mated strains is 1*107 per mL, 1 mL of the spore suspension is injected per seedling, and the tissue culture seedlings of sugarcane to be tested are tissue culture seedlings 35-45 cm in height. After Ustilago scitaminea is inoculated, origination is evident, operability is high, and pathogenicity test cycle is shortened;therefore, efficiency of screening Ustilago scitaminea pathogenic defective mutants is improved; technical support is provided for the identification and mechanism research on pathogenicity associatedgenes of Ustilago scitaminea; the method has important significance.

Description

technical field [0001] The invention belongs to the technical field of agriculture, and relates to a method for inoculating sugarcane tissue culture seedlings of sugarcane smut fungus. Background technique [0002] Sugarcane smut (Sugarcane smut) is an important sugarcane disease. Sugarcane smut was first reported in Natal, South Africa in 1877, and then the disease spread to countries in the eastern and western hemispheres. In my country, sugarcane smut was reported in Guangzhou sugarcane area in 1932, and then Guangxi, Yunnan, Fujian, Taiwan, Hainan and other provinces successively reported the occurrence of the disease. The occurrence of sugarcane smut was reported successively in the United States and Australia, and it spread rapidly and became the main sugarcane disease. So far, sugarcane smut has become a common sugarcane disease in various sugarcane producing areas in the world. The average incidence rate is 18%, and the incidence rate of ratoon cane is as high as 5...

AI Technical Summary

Problems solved by technology

The pathogenicity determination mainly adopts the method of artificial inoculation. First, mature sugarcane is harvested from the field, and it is potted in several sections using a knife. Due to the low germination rate of potted sugarcane, a large amount of sugarcane needs to be planted to ensure the inoculation material, which results in the need for a large number of sugarcane species. The material occupies a large area of ​​land; in addition, sugarcane seedlings need to grow for about 2 months before inoculation, or even longer, the pre-inoculation preparation time is relatively long, and it takes 2-4 months after inoculation to onset. This inoculation process takes a long time and is limited. Number of mutants tested

In addition, the inoculation results of S. cane smut are unstable and inaccurate, which wastes a lot of manpower, material resources, time and energy, thereby affecting the screening efficiency of pathogenic defect mutants of S. Slow progress in mechanistic research