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Application of pyrazine in attracting citrus psyllid and a citrus psyllid attractant

A technology of citrus psyllid and pyrazine, which is applied in the direction of attracting pests, application, and biocide, and can solve the problem of less compounds in citrus psyllid

Active Publication Date: 2021-04-06
GANNAN NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, currently few compounds are available to attract citrus psyllids

Method used

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  • Application of pyrazine in attracting citrus psyllid and a citrus psyllid attractant

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] (1) Use rubber tubes with a length of 30cm to connect the taste source bottles with different concentrations of pyrazine solutions at the different mouths of the four-necked flasks; the pyrazine solutions are prepared with ultrapure water, and the volume concentrations are 0% and 0.1% respectively , 1% and 10%, of which 0% is a blank control;

[0037] (2) Shade the four-necked flask containing the psyllid, put about 40 adults at the bottom of the flask at a time, and use the single-necked bottleneck of the four-necked flask as the boundary. The number of citrus psyllids at each bottle mouth and the total amount of four bottle mouth citrus psyllids were recorded every 10 minutes, and the induction efficiency was calculated. Each experiment was replaced with unused psyllids and repeated three times.

[0038] The statistics of the results are shown in Table 1.

[0039] Table 1 The induction efficiency (%) of different concentrations of pyrazine to citrus psyllid

[0040...

Embodiment 2

[0043] (1) Connect zone 1 and zone 2 of the six-arm olfactory instrument to the flavor source bottle, leave the remaining four arms empty and close the arm mouth, and zone 2 is set as the control zone;

[0044] (2) Turn on the vacuum pump, adjust the air flow rate of each arm of the six-arm olfactometer to 80mL / min, and wait until the air flow is stable;

[0045] (3) Put 1% pyrazine solution (solvent is ultrapure water) in the odor source bottle corresponding to area 1, and put an equal amount of ultrapure water in the odor source bottle corresponding to area 2 as a control.

[0046] Each experimental test was carried out head by head, and the number of times the psyllid entered different areas and the number of times it was in the area when it stayed motionless for 3 minutes was recorded as the test result of the citrus psyllid's chemotactic response to different volatile compounds. The test results are shown in Table 2 Show.

[0047] Table 2 Comparison of the number of time...

Embodiment 3

[0051] In insect cages with a size of 75*75*90 (cm), combined with yellow boards (size of 15*20 cm) to conduct insect trap experiments, the amount of drug coating on each yellow board was about 1mL. Pyrazine with a volume concentration of 0.1% (solvent is ultrapure water) was coated on the yellow board, and hung symmetrically with the yellow board without medicine in the insect cage. In the middle of the insect cage, put a plant of Murata japonica, and put about 20 psyllids in the cage, and add about 1mL of medicine on the yellow board the next day. Count the number of citrus psyllid adults on the yellow board every day, and the experimental results are shown in Table 3:

[0052] Table 3 0.1% pyrazine trap results

[0053] project first day the next day third day control 3 3 3 pyrazine 8 13 16

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Abstract

The invention relates to the field of plant disease and insect pest control, and provides an application of pyrazine in attracting citrus psyllids and an attractant for citrus psyllids. Pyrazine is a volatile compound, and its volatilized unique smell can attract citrus psyllids, and it can achieve high-efficiency attracting citrus psyllids without adding other attractant substances; the citrus psyllid attractant provided by the invention includes pyrazine and solvent , the attractant has a good attracting effect on citrus psyllids, has a long acting time and is convenient to use. The results of the examples show that when pyrazine is used to lure citrus psyllids, the induction efficiency can reach 43.19% under the condition of a volume concentration of 0.1%.

Description

technical field [0001] The invention relates to the technical field of plant disease and insect pest control, in particular to the application of pyrazine in attracting citrus psyllids and an attractant for citrus psyllids. Background technique [0002] Citrus psyllid (Diphorin itri Kuwym) is the main pest of citrus fruit trees in the new shoot stage, and is also the main carrier of Huanglongbing pathogenic bacteria. Feathering. The young shoots of the damaged host may wither and deform the new shoots. The psyllids also secrete white honeydew that adheres to the branches and leaves, which can cause the occurrence of soot disease. [0003] For the control of citrus psyllids, a variety of methods have been established at present, among which chemical control mainly uses pesticides, which can effectively control citrus psyllids by actively applying insecticides, but this method will cause drug resistance, environmental pollution, pesticides Residues, harm to natural enemies ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N43/60A01P19/00A01M1/02A01M1/04
CPCA01M1/02A01M1/04A01N43/60
Inventor 林妗蓓彭婷
Owner GANNAN NORMAL UNIV
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