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A rapid quantitative detection method for immunochromatographic test paper card

A technology of immunochromatographic test strips and quantitative detection methods, which is applied in the field of biomedical detection, can solve problems such as large calculations, and achieve the effects of easy implementation, increased operating speed, and reduced calculations

Active Publication Date: 2020-06-02
武汉纳达康生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The existing image processing immunochromatography quantitative detection methods all include steps that require a very large amount of calculation, such as building a deep belief network or edge detection, which are difficult to implement on ordinary embedded instruments in practical applications

Method used

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  • A rapid quantitative detection method for immunochromatographic test paper card
  • A rapid quantitative detection method for immunochromatographic test paper card
  • A rapid quantitative detection method for immunochromatographic test paper card

Examples

Experimental program
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Effect test

Embodiment 1

[0051] This embodiment introduces in detail the rapid quantitative detection method of the immunochromatography test paper card in the present invention by processing the colloidal gold immunochromatography test paper card of vomitoxin.

[0052] The specific experimental process comprises the following steps: (1) dripping the urine sample to be tested on the sample hole 2 of the vomitoxin colloidal gold immunochromatographic test paper card, the immunochromatographic test paper card is as follows: figure 2 As shown, it includes immunochromatography test paper card 1 to be tested, sample hole 2, observation window 3, quality control line 4, detection line 5, reference area 6 composed of two standard color blocks, and the black standard color block is used as a dark field Benchmark, the white standard color block is used as the bright field benchmark, and after waiting for 3 minutes, the image is collected by a digital camera to capture the original image of the test paper card;...

Embodiment 2

[0068] This example introduces in detail the rapid quantitative detection method of the immunochromatography test card in the present invention by processing the aflatoxin B1 latex particle immunochromatography test card.

[0069] The specific experimental process includes the following steps: (1) Add the liquid to be tested dropwise on the sample hole of the aflatoxin B1 latex particle immunochromatography test paper card, wait for 3 minutes, and collect the image through the scanner to capture the original color of the test paper card. image;

[0070] (2) First pass the original image through the formula (Gray=0.15*R+0.8*G+0.05*B (green light absorption is the best, blue light absorption is the worst, and red light absorption is average, so y is the largest, z is the smallest, and x Take the number between the two)) to convert to a grayscale image, such as Figure 5 Middle (a); then compress the grayscale image into a one-dimensional original curve, such as Figure 5 In (b...

Embodiment 3

[0086] This embodiment introduces in detail the rapid quantitative detection method of the immunochromatography test card in the present invention by processing the zearalenone colloidal gold immunochromatography test card.

[0087] The specific experimental process includes the following steps: (1) Add the liquid to be tested dropwise on the sample hole of the zearalenone colloidal gold immunochromatography test paper card, wait for 5 minutes, and collect images through the camera to capture the original color of the test paper card. image;

[0088] (2) First pass the original image through the formula (Gray=0.2*R+0.7*G+0.1*B (green light absorption is the best, blue light absorption is the worst, and red light absorption is average, so y is the largest, z is the smallest, and x Take the number between the two)) to convert to a grayscale image, such as Figure 7 Middle (a); then compress the grayscale image into a one-dimensional original curve, such as Figure 7 In (b), th...

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Abstract

The invention discloses a rapid quantitative detection method for immunochromatographic test paper cards. The method includes the following steps: (1) obtaining an original image; (2) converting the original image into a grayscale image, and compressing the grayscale image into a one-dimensional original curve; (3) performing baseline correction on the one-dimensional original curve to obtain a corrected curve; (4) performing eigenvalue calculation on the corrected curve; and (5) calculating the concentration of a to-be-tested object according to an eigenvalue. The provided method can rapidlyobtain the concentration of the to-be-tested object, is very small in calculated quantity without setting parameters, and can be easily realized on common embedded instruments, so that the purpose ofrapidly, automatically and quantitatively detecting immunochromatographic test paper cards can be achieved.

Description

technical field [0001] The invention belongs to the technical field of biomedical detection, and more specifically relates to a rapid quantitative detection method of an immunochromatographic test paper card. Background technique [0002] Immunochromatography is a rapid immunoassay technology developed in recent years. Its basic principle is the interaction between antibodies and antigens. Immunochromatography technology has strong specificity, accurate detection results, simple equipment operation, and rapid measurement, which fully meets the requirements of instant detection. At present, immunochromatography technology has been widely used in medical testing, food safety testing, drug testing, environmental testing and other fields. [0003] With the continuous improvement of laboratory personnel's requirements for immunoassay, immunochromatography technology has developed from the initial qualitative or semi-quantitative detection to the current quantitative detection. ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/558
CPCG01N33/558
Inventor 杨海郭文胜张德军杨祥良
Owner 武汉纳达康生物科技有限公司
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