Mold and method for preparing silt soil sample based on microbial grouting

Abstract

The present invention relates to a mold for preparing a silt soil sample based on microbial grouting. The mold comprises two templates that are in detachable connection; wherein each template comprises a bottom plate, an inner curved plate and an outer curved plate, a plurality of first circular holes are arranged on sidewall of the inner curved plate, and a plurality of second circular holes arearranged in the bottom plate, and a steel wire mesh is arranged on inner surface of the inner curved plate; a filter unit is disposed in an enclosed space on the inner curved plate above the bottom plate. The invention also provides a method for preparing the silt soil sample based on microbial grouting, the method comprises following steps: selecting a mold; culturing microorganism; measuring urease activity; putting the soil into the mold; and injecting microbial liquid and a nutrient solution along the periphery. The mold and method can be applied to silt soil with small permeability coefficient, passes the microbial liquid through the plurality of first circular holes as grouting channels to realize multi-dimensional and multipoint filling inside the soil, can effectively solve grouting uniformity without disturbing the soil, and ensure grouting effect and improving efficiency.

Description

technical field [0001] The invention relates to the field of biological sample preparation, in particular to a mold and a method for preparing silt soil samples based on microbial grouting. Background technique [0002] Studies have shown that microbe-induced calcium carbonate deposition and cementation of loose soil is a green and environmentally friendly new method of soil consolidation. Its main mechanism is to solidify soil through microbial grouting. For coarse-grained soil with large pores, the treatment process is relatively simple and convenient and the curing effect is good. For fine-grained soil with small pores, the treatment process is often more difficult and the treatment effect is poor; According to the existing research results, the research object of microbial solidification method is mainly concentrated on pure sandy soil, and the treatment method has been relatively mature and the treatment effect is better. Difficulties that need to be further broken thr...

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Problems solved by technology

[0002] Studies have shown that microbe-induced calcium carbonate deposition and cementation of loose soil is a green and environmentally friendly new method of soil consolidation. Its main mechanism is to solidify soil through microbial grouting. For coarse-grained soil with large pores, the treatment process is relatively simple and convenient and the curing effect is good. For fine-grained soil with small pores, the treatment process is often more difficult and the treatment effect is poor; According to the existing research results, the research object of microbial solidification method is mainly concentrated on pure sandy soil, and the treatment method has been relatively mature and the treatment effect is better. Difficulties to be further broken through in research

[0003] At present, the focus of indoor experiments on silt is how to treat the samples so that the microorganisms can be evenly distributed inside the soil samples, mainly for pressure-free seepage. Although this method is simple and easy to operate, it is not suitable for the For fine-grained soil, the grouting period is long, and the effect is not obvious; there is pressure seepage, although the disturbance to the soil is reduced, but the actual operation is difficult and the cost is high; Experimental results

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