Method for simultaneously selecting and breeding pepper male sterile line and homozygous restorer gene line by using recovery gene linkage marker
A male sterile line and gene restoration technology, applied in the field of breeding, to achieve the effect of reducing the number of self-breeding generations, reducing the impact of environmental factors, and being easy to obtain
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[0045]The invention uses the capsicum male sterile three-line hybrid as the basic material, and constructs the F2 generation segregation population in a 40-mesh isolation net room. According to the results of the field fertility survey at the flowering stage, the sterile pool and the fertile pool were constructed, and the polymorphism screening was carried out using the pepper restorer gene linkage marker. As a result of electrophoresis in the fertile pool, when the band type is two bands at a molecular weight of 82bp, one molecular weight is different from the size in the sterile pool, and the other is the same, the restoration gene linkage marker can be used in the F2 population The identification of homozygous individual plants at the restorer gene locus was carried out. Then, all fertile plants in the F2 population were screened by molecular markers, and the plants with only one band and different molecular weight from those in the sterile pool were selected, that is, the ...
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