A kit for detecting genetic molecular markers associated with susceptibility to endometrial cancer

Abstract

The invention belongs to the technical field of molecular biology and biomedicine and discloses an endometrial cancer susceptibility associated genetic molecular marker detection kit. A nucleotide sequence of a genetic molecular marker is shown as SEQ ID NO.1, and single nucleotide polymorphism C>T exists at 501th site, namely a basic group at the 501th site of the sequence can be a basic group Cand a basic group T. The kit comprises two pairs of specific PCR primers designed on the basis of the single nucleotide polymorphism genetic molecular marker of an NANOG gene, the genetic molecular marker is closely associated with endometrial cancer susceptibility, single nucleotide polymorphism in the NANOG gene can be effectively detected by means of the PCR technology, and further, endometrialcancer susceptibility can be predicted, so that important clinical significance to screening of endometrial cancer susceptible population, detection of high-risk individuals and early prediction, early diagnosis and individual treatment of the endometrial cancer is achieved.

Description

technical field [0001] The invention relates to the technical fields of molecular biology and biomedicine, in particular to a kit for detecting endometrial cancer susceptibility based on the SNP site of the NANOG gene. Background technique [0002] Endometrial cancer is one of the most common gynecological tumors in the world. Its incidence has been increasing over the past few decades and tends to be younger, especially in developed countries. In recent years, as the global obesity rate has increased, the incidence of endometrial cancer in China has also increased, with 63,400 newly diagnosed cases in 2015. According to the FIGO 2009 classification, 80% of endometrial cancers are clinical stage I-II when diagnosed, the 5-year survival rate of stage I is 89.6%, that of stage II drops to 78.3%, that of stage III is 61.9%, and that of stage IV is 21.1%. Although endometrial cancer diagnosed early can be cured with surgery plus adjuvant radiotherapy or chemotherapy, approxima...

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Problems solved by technology

However, these studies only stayed at the level of protein expression and did not detect at the level of genetic background at the DNA level. Changes in protein levels are often the result of malignant transformation of cells, which are late events at the molecular level, and cannot be detected at the stage of disease or even at the onset of the disease. Detection and warning before it happens

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