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Method for identifying salt tolerance of different wheat varieties in germination period

An identification method and a technology of germination period, applied in the field of agricultural technology application, can solve the problems of single measurement index, small salt concentration gradient, and failure to reflect the comprehensive germination status of crops, etc., achieve strong system, overcome poor comprehensiveness, and accelerate salt tolerance The effect of the breeding process

Active Publication Date: 2019-08-16
QINGDAO AGRI UNIV
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Problems solved by technology

[0004] At present, the methods for identifying the salt tolerance of crops are: the patent "a method for identifying the salt tolerance of seed plants at the germination stage", application number: 201710138050.X; the patent "a method for identifying the salt tolerance of cotton seeds", application number: 201811460623.1 ;Patent "A Method for Identifying Salt Tolerance in Wheat Germination Stage", Application No.: 201410006260.X; These existing technologies have a single measurement index, which is somewhat one-sided in evaluating the salt tolerance of wheat, and the salt concentration gradient is small , has certain limitations, and cannot reflect the comprehensive germination status of crops under multi-level salt stress conditions

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  • Method for identifying salt tolerance of different wheat varieties in germination period
  • Method for identifying salt tolerance of different wheat varieties in germination period
  • Method for identifying salt tolerance of different wheat varieties in germination period

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Embodiment

[0040] A method for identifying salt tolerance of different wheat varieties during germination, the steps are as follows:

[0041] (1) Determination of relative germination rate and relative germination potential of wheat to be tested

[0042]Add 4.68g, 9.36g, and 14.04g NaCl to each liter of distilled water to prepare 80mmol / L, 160mmol / L, and 240mmol / L NaCl solutions, and use 0mmol / L, 80mmol / L, 160mmol / L, and 240mmol / L NaCl solutions respectively Treat the wheat seeds to be tested, place them in a light incubator at a constant temperature of 21°C, and cultivate them in the dark for 24 hours, then place them at room temperature, measure the germination rates of the control group and the treatment group three days later, measure the germination potential after seven days, and calculate The relative germination rate and relative germination potential of the wheat varieties to be tested under the treatment of 0mmol / L, 80mmol / L, 160mmol / L, and 240mmol / L NaCl solution:

[0043] Ge...

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Abstract

The invention discloses a method for identifying salt tolerance of different wheat varieties in a germination period and belongs to the field of application of agricultural technologies. According tothe method for identifying salt tolerance of different wheat varieties in the germination period, the germination rate, germination potential, root length, first leaf length, coleoptile length, bud dry weight, seedling fresh weight, root dry weight and dry substance transport rate of wheat seeds with four different concentrations are measured, the relative value and the weighted average are calculated, the weighted average of all indexes is imported into a comprehensive index calculation formula, and a comprehensive salt tolerant index is calculated. The method for identifying the salt tolerance of different wheat varieties in the germination period is high in systematicness, simple and convenient to operate, wide in applicability and high in comprehensiveness, and is more accurate in evaluation of the tolerance of the wheat in the germination period and has more reliable results than a salt-tolerant membership function value method.

Description

technical field [0001] The invention belongs to the field of agricultural technology application, in particular to a method for identifying the salt tolerance of different wheat varieties in germination stage. Background technique [0002] Soil salinization and secondary salinization have become resource constraints for the sustainable development of irrigated agriculture in the world. Among the existing cultivated land in the world, there are about 340 million hm 2 saline soil and 560 million hm 2 The soda saline soil accounts for 60% of the total cultivated land area, and the saline-alkali land in my country accounts for about 20% of the cultivated land area. Soil salinization and secondary salinization have caused serious losses to agricultural production, which is one of the ecological crises faced by human beings. Domestic and foreign scholars' research on crops' salt-tolerance has played an important role in enhancing plant's salt-tolerance, increasing crop yield an...

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Application Information

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IPC IPC(8): A01C1/02
CPCA01C1/025
Inventor 郭卫卫韩丽萍张玉梅
Owner QINGDAO AGRI UNIV
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