An integrated bioreactor for adherent cell culture

Abstract

The invention provides an integrated bioreactor for culturing adherent cells, the bioreactor includes a control system, a reactor body and a cell culture unit connected in sequence, and the control system, the reactor body and the cell culture unit The inside is connected; the control system is provided with a gas pressure balance hole and a drip hole, and the cell culture unit is provided with a central diversion hole and a liquid level monitoring hole, and the gas pressure balance hole and the liquid level monitoring hole The liquid level sensor is formed by connecting through pipes, and the bioreactor also includes a cell suspension bottle, one end of the central diversion hole is connected with the drip hole through a pipe, and the other end is connected with the cell suspension bottle through a pipe. The present invention successfully exchanges and circulates the medium of the cell culture unit and the control system, and successfully realizes the unified regulation of the pH value and dissolved oxygen value of the culture medium of each unit; also realizes the control of the laminar flow shear force and liquid Face height control.

Description

technical field [0001] The present invention relates to the technical field of bioreactors, and more particularly, to an integrated bioreactor for adherent cell culture. Background technique [0002] Bioreactors are in vitro culture expansion devices that can well mimic the internal environment of organisms to provide the required nutrients, physical environment and chemical environment for the growth of different cells. Therefore, bioreactors have great development prospects and potential in drug production, tissue engineering and regenerative medicine. [0003] In the bioreactor, the flowable culture medium makes the nutrients in the culture medium more evenly distributed, so that the cells can absorb the nutrients well for growth and excrete metabolic wastes immediately. At present, bioreactors can be divided into many types according to different culture methods. According to the flow of culture fluid, it can be divided into perfusion bioreactor and hybrid bioreactor. ...

AI Technical Summary

Problems solved by technology

The problem with perfusion bioreactors is that the shear force of the flow field at the inlet of the culture medium is relatively large, which cannot provide a suitable growth environment for cells.

In addition, cells are subjected to shear forces at the bubble rupture, resulting in reduced cell viability

The current multi-layer plate bioreactor directly perfuses the culture medium into the multi-layer plate structure, resulting in an unevenly distributed flow field shear force environment, which inhibits the growth of cells.

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