SSR molecular marker associated with cotton short stalk and high lint percentage
A molecular marker and lint technology, applied in biochemical equipment and methods, microbial determination/inspection, DNA/RNA fragments, etc., can solve the problems of difficulty in cotton yield, environmental pollution, time-consuming and labor-intensive, and achieve rapid and accurate identification. stability, speed up the breeding process, increase the selection efficiency and the effect of breeding speed
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[0051] Example 1. Determination of chromosome segments related to cotton short stem and high lint traits and development of related molecular markers
[0052] The low-stalk high-grain cotton in the present invention is cultivated through the following steps:
[0053] Using upland cotton 86-1 as the donor, crossed with abnormal male parent cotton to obtain triploid F 1 (A 1 D 1 B 1 ), hexaploid F is obtained by treating the tender axillary buds of triploid seedlings with 0.15% colchicine 1 (A 1 A 1 D 1 D 1 B 1 B 1 );
[0054] Using the hexaploid as the female parent, crossed with the parent Su 8289 to obtain Pentaloid A 1 A 1 D 1 D 1 B 1 , Planted all the received seeds and got 97 individual plants;
[0055] The obtained pentaploid is backcrossed with Su 8289, and BC is obtained 2 F 1 All the seeds were planted, and 384 individual plants were obtained, with 50 recombination types, 36 of which were fertile;
[0056] Will BC 2 F 1 All individuals of and backcrossed with Su 8289 and obtaine...
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