Leonurus artemisia extract, application thereof to fish feed, fish feed and preparation method of fish feed
A kind of motherwort extract and technology of motherwort, applied in the field of fish feed, can solve the problems of insignificant growth-promoting effect, low utilization rate, and unclear active ingredients, so as to improve anti-superoxide anion activity, improve feed efficiency, and benefit fish The effect of class growth
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Embodiment 1
[0059] Obtain 1000 g of the aerial part of Motherwort, dry it in a blast drying oven at 50° C. to constant weight, crush it through a 18-mesh sieve, and obtain Motherwort powder.
[0060] Take motherwort powder 400g, mix motherwort powder and petroleum ether according to the ratio of 1:7-9, rotate and stir at 800 rpm for 6 hours; filter the mixed solution to obtain a clear filtrate and residue, repeat the extraction of the residue 3 times and combine The filtrate was obtained to obtain residue I; the filtrate was distilled under reduced pressure to constant weight to remove petroleum ether, and the extract of motherwort petroleum ether was obtained. Mix the residue I after extraction of motherwort petroleum ether with ethyl acetate at a ratio of 1:7-9, and rotate and stir at 800 rpm for 6 hours; filter the mixture to obtain a clear filtrate and residue, and repeat the extraction of the residue 3 times The filtrates were combined to obtain residue II; the filtrate was distilled...
Embodiment 2
[0072] In order to explore the effect of motherwort extract as a fish feed additive on high-density culture of freshwater fish, the following experiments were carried out:
[0073] 1. To study the effects of stocking density changes on fish body weight, food intake and feed efficiency.
[0074] A number of crucian carp fry were purchased locally, and after being temporarily raised in the breeding room for a week, 720 crucian carp fry with a body weight of 8.0±0.2g were selected and randomly divided into 8 treatment groups, each with 4 fish tanks, and the number of fish tails in the 8 treatment groups were respectively : 5, 10, 15, 20, 25, 30, 35 and 40 tails / cylinder. Among them, the shape, size and color of each fish tank are the same, the size is 30cm×30cm×40cm, and a built-in 800L / h flow aerator. Tap water is used as the water source, and the water volume in the tank is 31L. The breeding room temperature is controlled at 22±2°C; the aerator continuously supplies oxygen; t...
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