Method for enhanced extraction and repair of moderately cadmium contaminated farmland through intercropping of oilseed rape and sedum alfredii
A technology of rapeseed and farmland, applied in the field of phytoremediation of soil heavy metal pollution, can solve problems such as difficult large-scale planting, and achieve the effect of improving the modification effect, good yield level, and easy operation
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[0036] The preparation method of the endophyte I fermentation liquid is as follows: inoculate the endophyte I to be tested in a sterile LB liquid medium, cultivate it at 30°C and 200 rpm in a fermenter for 24 hours, and then dilute it with sterile water Fermentation broth to OD value of about 1.0, made into a concentration of 1 × 10 8 CFU / mL of bacterial suspension is ready for use. The components of the medium are: tryptone 10g / L, yeast extract 5g / L, sodium chloride 10g / L, pH 7.2, sterilized at 121°C for 20min.
[0037] The preparation method of the endophyte II fermentation liquid is as follows: inoculate the endophyte II to be tested in a sterile LB liquid medium, cultivate it at 30°C and 200 rpm in a fermenter for 24 hours, and then dilute it with sterile water Fermentation broth to OD value of about 1.0, made into a concentration of 1 × 10 8 CFU / mL of bacterial suspension is ready for use. The components of the medium are: tryptone 10g / L, yeast extract 5g / L, sodium chl...
Embodiment 1
[0039] 1. Test location and soil physical and chemical properties
[0040] A heavy metal-contaminated farmland in Changxing County, Huzhou City (test point A), a heavy metal-contaminated farmland in Qujiang District, Quzhou City (test point B), and a heavy metal-contaminated farmland in Ninghai County, Ningbo City (test point C) were used as test fields.
[0041] The basic physical and chemical properties and heavy metal content of the soil at each test site are shown in Table 1.
[0042] Table 1 The basic physical and chemical properties of the soil at each test point
[0043]
[0044]
[0045] 2. Test steps
[0046] (1) At the end of September of the first year, seeds of rapeseed (Lvsheng No. 1) were sown in the field next to the soil to be repaired, and seedlings were raised; in October of the first year, the herbicide glyphosate was sprayed on the soil to be repaired , and then carry out normal plowing, soil preparation, and furrows; when the rapeseed seedlings grow ...
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