Alcohol quantitative analysis method based on double-enzyme-inorganic nanoflower composite material
A technology of inorganic nano and composite materials, applied in the field of quantitative analysis, can solve problems such as low operational stability, narrow linear operating range, low sensitivity, etc., and achieve improved enzyme activity and stability, special material morphology, and high specific surface area Effect
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[0025] A method for quantitative analysis of alcohol based on double enzyme-inorganic nano flower composite material, comprising the following steps:
[0026] (1) Preparation of dual enzyme-inorganic nanoflower composite materials: such as figure 1 As indicated, 15 μL of alcohol oxidase (AOX) solution 1.0 mg / mL and horseradish peroxidase (HRP) 1.0 mg / mL were added to 1.41 mL of phosphate buffered saline (PBS), and then 168 μL of 100 mM CaCl 2 Add the solution to the above solution, mix it evenly, let it stand still, incubate at 30°C for 48 hours, take it out, then centrifuge at 10000r / min for 15min, remove the supernatant, then add 150μL of PBS, mix well, and you can get The dual enzyme-inorganic nanoflower composite (AOHNF) required for the experiment.
[0027] Such as figure 2 As shown, the dual enzyme-inorganic nanoflower composite material is characterized by SEM. The dual enzyme-inorganic nanoflower composite material is a uniform nanoflower-shaped ball with an averag...
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