A kind of sulfated jelly jelly polysaccharide and preparation method thereof
A technology of jelly grass and sulfation, which is applied in the direction of anti-toxic agents and drug combinations, can solve the problem of low biological activity of polysaccharides, and achieve the effect of improving the protective effect
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[0020] 1. Extraction of jelly jelly polysaccharide
[0021] Soak jelly grass with 9 times the volume of 80% ethanol for 20 hours after crushing, and air-dry the residue in a cool place. After pretreatment, add 15 times the volume of the jelly jelly, extract with boiling water for 2 hours, filter the supernatant with 120-mesh gauze, repeat the extraction twice from the filter residue, combine the filtrates, concentrate and centrifuge to obtain the jelly jelly crude polysaccharide solution.
[0022] 2. Purification of jelly jelly polysaccharide
[0023] Use 80% ethanol to precipitate the crude polysaccharide solution at 4°C for 20 hours, repeat it 4 times, and put it on the D301 macroporous resin chromatography column, elute the resin column with 0.2M NaCl solution, control the flow rate at 1.8mL / min, collect eluent. Concentration, dialysis to remove small molecular substances, and freeze-drying to obtain neutral and uniform jelly polysaccharide (MP).
[0024] 3. Sulfation mo...
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