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Microbial water quality improver and preparation method thereof

A water quality improver and microbial technology, applied in biological water/sewage treatment, granular microbial carrier treatment, chemical instruments and methods, etc., can solve the problems of difficult survival of microbial strains, poor water quality improvement effect, loss, etc., and achieve improvement Effect of improving water quality, improving resistance, and high survival rate

Active Publication Date: 2019-07-12
SHANDONG INST FOR FOOD & DRUG CONTROL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The invention provides a microbial water quality improver and a preparation method thereof to solve the problem that the microbial strains in the microbial water quality improver are difficult to survive and the loss is serious, resulting in poor water quality improvement effect

Method used

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Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0025] The preparation method of microbial water quality improver provided by the invention comprises the following steps:

[0026] Step S001: Take glucose, xylan and mannose with a molar ratio of 1:0.5:0.5, add deionized water to dissolve, make the concentration of glucose 0.5mol / L, and then add FeCl 3 , NaCl, MgCl 2 , CaCl 2 Mixed, glucose with FeCl 3 , NaCl, MgCl 2 , CaCl 2 The molar ratio is 1:0.1:0.1:0.1:0.1. The mixed solution was transferred to a reaction kettle, hydrothermally reacted in a vacuum oven at 180°C for 12 hours, washed and filtered, and dried at 80°C for 12 hours to obtain carbon microspheres.

[0027] Step S002: Configure 100 mL of beef extract peptone agar plate medium according to the medium formula, and pour the plates separately after sterilization.

[0028] Step S003: Inoculate the bacillus on the plate with aseptic technique, spread evenly, and select appropriate three dilutions to inoculate on the beef extract peptone agar medium to obtain the...

Embodiment 1

[0037] A kind of microbial water quality improving agent that present embodiment 1 provides, its preparation method comprises the following steps:

[0038] Step S101: Take glucose, xylan and mannose with a molar ratio of 1:0.5:0.5, add deionized water to dissolve, make the concentration of glucose 0.5mol / L, and then add FeCl 3 , NaCl, MgCl 2 , CaCl 2 Mixed, glucose and FeCl 3 , NaCl, MgCl 2 , CaCl 2 The molar ratio is 1:0.1:0.1:0.1:0.1. The mixed solution was transferred to a reaction kettle, hydrothermally reacted in a vacuum oven at 180°C for 12 hours, washed and filtered, and dried at 80°C for 12 hours to obtain carbon microspheres.

[0039] Step S102: Configure 100 mL of beef extract-peptone agar plate medium according to the medium formula, and pour the plates separately after sterilization.

[0040]Step S103: Inoculate Bacillus subtilis, Bacillus megaterium, Bacillus pumilus, and Bacillus licheniformis on the plate with aseptic technique, spread evenly, and select ...

Embodiment 2

[0046] Another kind of microbial water quality improver that present embodiment 2 provides, its preparation method comprises the following steps:

[0047] Step S201: Take glucose, xylan and mannose with a molar ratio of 1:0.5:0.5, add deionized water to dissolve, make the concentration of glucose 0.5mol / L, and then add FeCl 3 , NaCl, MgCl 2 , CaCl 2 Mixed, glucose and FeCl 3 , NaCl, MgCl 2 , CaCl 2 The molar ratio is 1:0.1:0.1:0.1:0.1. The mixed solution was transferred to a reaction kettle, hydrothermally reacted in a vacuum oven at 180°C for 12 hours, washed and filtered, and dried at 80°C for 12 hours to obtain carbon microspheres.

[0048] Step S202: Configure 100 mL of beef extract-peptone agar plate medium according to the medium formula, and pour the plates separately after sterilization.

[0049] Step S203: Bacillus subtilis was inoculated on the plate with aseptic technique, spread evenly, and inoculated on beef extract peptone agar medium at three appropriate d...

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Abstract

The invention discloses a microbial water quality improver and a preparation method thereof. The preparation method comprises the following steps: (1) preparation of carbon microspheres; (2) preparation of a plate medium; (3) inoculation; (4) plate culturing; 5) shake-flask culturing; (6) preparation of a bacterium suspension; and (7) preparation of the globular microbial water quality improver. TGlucose, polyxylose and mannose are used as a mixed carbon source to prepare the high-quality carbon microspheres as a carrier of a strain, the strain is reasonably fixed, the survival rate is high, and the formation of stable colonies is facilitated. Polyvinyl alcohol, sodium alginate and silica are used to immobilize the colonies, so the degradation efficiency of Bacillus spp on COD is ensured,and the resistance of Bacillus spp colonies to toxic and harmful substances is improved, thereby the water quality improvement effect of the culturing waters is improved.

Description

technical field [0001] The invention relates to the technical field of water quality improvement, in particular to a microbial water quality improver and a preparation method thereof. Background technique [0002] With the rapid development of the aquaculture industry, unreasonable farming behaviors and increasing scale of farming have increasingly serious adverse effects on water quality. A good ecological environment in aquaculture waters is a necessary condition for the survival and reproduction of aquatic products. In the process of artificial aquaculture, when the water quality in ponds and other aquaculture waters is poor, it will not only affect the normal growth and reproduction quality of aquatic products, but even It can also cause the death of aquatic products, cause great economic losses, and indirectly affect human health. Solving the water quality problems of artificial aquaculture water bodies has become an inevitable requirement for the development of green,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C02F3/34C02F103/20
CPCC02F3/348C02F2003/003C02F2103/20C02F2305/06
Inventor 翟清燕任易婕霍胜楠郭颖慧郑世超孟静
Owner SHANDONG INST FOR FOOD & DRUG CONTROL
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