Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Anti-calf escherichia coli F41 pilus antigen hyperimmune egg yolk antibody IgY preparation method and application

A technology of Escherichia coli and egg yolk antibody, applied in the agricultural field, can solve the problems of insufficient purity, reduced effect of repeated use, and low coverage of Escherichia coli

Pending Publication Date: 2019-06-21
INNER MONGOLIA UNIV FOR THE NATITIES
View PDF2 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The purity of anti-animal Escherichia coli hyperimmune egg yolk antibody IgY extraction is not high enough; Escherichia coli serotypes are complex and diverse, and the coverage of IgY for serotypes on pathogenic Escherichia coli is not high; animals after inoculation are prone to produce anti-IgY animal Escherichia coli Antibodies that are highly immune to egg yolk antibodies, the effect of repeated use is reduced

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Anti-calf escherichia coli F41 pilus antigen hyperimmune egg yolk antibody IgY preparation method and application
  • Anti-calf escherichia coli F41 pilus antigen hyperimmune egg yolk antibody IgY preparation method and application
  • Anti-calf escherichia coli F41 pilus antigen hyperimmune egg yolk antibody IgY preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0057] (1) Isolation and identification of pathogenic bacteria; the specific steps are:

[0058] (a) Inoculate the collected calf diarrhea feces on ordinary agar medium, MacConkey medium and eosin methylene blue medium respectively, place them in a constant temperature incubator at 37°C for continuous culture for 24 hours, select a single colony for staining and microscopic examination, Then carry out purification culture;

[0059] (b) select the calf pathogenic Escherichia coli bacterial strain that isolates, inoculate respectively nutrient broth substratum culture, the preparation bacteria content is 10 9 CFU / mL suspension was inoculated into the mice intraperitoneally, each mouse was inoculated with 0.2mL, a total of 5 mice, and a group of blank control mice were inoculated with sterile nutrient broth, each mouse was inoculated with 0.2mL, and a total of 5 mice were inoculated. Five mice were isolated and observed for 5 days after inoculation, and the death of the mice was...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
concentrationaaaaaaaaaa
Login to View More

Abstract

The invention relates to the technical field of agriculture, in particular to an anti-calf escherichia coli F41 pilus antigen hyperimmune egg yolk antibody IgY preparation method and application. Themethod includes steps: (1) pathogenic bacterium separation and identification; (2) pilus gene identification and screening; (3) pilus antigen extraction and purification; (4) egg yolk sterile separation; (5) antibody purification. Clear thinking is realized, by adoption of calf escherichia coli F41 pilus as an antigen for immunization of egg-laying hens, the egg-laying hens generate an anti-pilusantibody, hyperimmune eggs are collected, egg yolks are separated, and an antibody extracted from the egg yolks is a hyperimmune egg yolk antibody (IgY), and the hyperimmune egg yolk antibody is applied to treatment and prevention of colibacillosis of calves.

Description

technical field [0001] The invention relates to the field of agricultural technology, in particular to a preparation method and application of an anti-calf Escherichia coli F41 pili antigen hyperimmune egg yolk antibody IgY. Background technique [0002] Calf Escherichia coli is a common bacterial infectious disease, and calves have a high mortality rate after the onset. There are many serotypes of pathogenic Escherichia coli, which brings difficulties to the prevention of this disease. Generally, antibiotics are selected for treatment, but antibiotics often develop drug resistance during treatment, or cause the beneficial bacteria in the intestinal tract of calves to degenerate. damage and delay treatment. In particular, the promulgation and implementation of the anti-antibiotic ban has reduced the number of antibiotics that can be used clinically, which has brought difficulties to the treatment of calf colibacillosis. [0003] Animals are generally stimulated by pathogen...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C07K16/12C07K16/02C12N1/20C12N1/02C12N15/10C12N15/66C07K14/245A61K39/40A61P31/04A61P1/12C12R1/19
CPCY02A50/30
Inventor 刘锴马德慧白浩丞石芸史量全王永强尹永勤李天旭郭双张荣荣李钗周虹
Owner INNER MONGOLIA UNIV FOR THE NATITIES
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com