Preparation technology of DNA (DesoxyriboNucleic Acid) for treating pneumonia

Abstract

The invention discloses a preparation technology of DNA (DesoxyriboNucleic Acid) for treating pneumonia and belongs to the technical field of separation purification of natural drugs. The preparationtechnology comprises the following steps: soaking macroporous cation exchange resin with acid fluid, completely draining the acid fluid and flushing with water; then soaking with lye and flushing withthe water; then soaking the macroporous cation exchange resin with the acid fluid again, flushing with deionized water and replacing with low carbon alcohol; adding phosphoglyceride into the low carbon alcohol, adding alkali for reacting, carrying out suction filtration, washing with the low carbon alcohol, and adjusting the pH to weak basicity, carrying out vacuum concentration, cooling, transferring to a separating funnel, standing and separating out a lower low carbon alcohol phase; loading the phosphoglyceride and phenolic resin on a column, carrying out column adsorption on alcoholysis mixed liquid, washing the resin with the low carbon alcohol, purifying and eluting the resin with pure water; decolorating by using a decoloring agent and carrying out vacuum concentration to obtain afinished product. The preparation technology has the advantages that technological steps are short and the requirements of industrial production are met; resources are saved and the cost is reduced; the total yield of a finished product reaches 65.47 to 86.08 percent, the external standard content reaches 99 percent or above and the optical purity reaches 99 percent.

Description

technical field [0001] The invention belongs to the technical field of separation and purification of natural medicines, and in particular relates to a preparation process of deoxyribonucleic acid for treating pneumonia. Background technique [0002] The preparation methods of deoxyribonucleic acid include solvent extraction, precipitation, recrystallization and column chromatography. The earliest purification and extraction method is to extract and purify new deoxyribonucleic acid from bovine pancreas. In 1954, U.S. Patent No. 2,864,848 disclosed a mercury salt precipitation method, which was to precipitate by-products in the form of mercury salts, and remove excess mercury ions by hydrogen sulfide and barium carbonate, but it was difficult to fully remove mercury ions, due to the residual Reactive PC, therefore, need to add calcium salt to precipitate new deoxyribonucleic acid to remove mercury ions, and finally use resin to remove calcium salt. It can be seen that the m...

AI Technical Summary

Problems solved by technology

In 1954, U.S. Patent No. 2,864,848 disclosed a mercury salt precipitation method, which was to precipitate by-products in the form of mercury salts, and remove excess mercury ions by hydrogen sulfide and barium carbonate, but it was difficult to fully remove mercury ions, due to the residual Reactive PC, so it is necessary to add calcium salts to precipitate new DNA to remove mercury ions, and finally use resin to remove calcium salts

It can be seen that the efficiency of this method is low, the process is more complicated, and the final yield is not ideal

Subsequently, Brockerhoff used the recrystallization method to prepare a new type of pure deoxyribonucleic acid, but the catalyst needs to be removed, and the product is recrystallized and purified. The whole process is complicated and costly, and it is not suitable for commercial scale production.