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Test paper applicable to quantitative detection of peripheral blood immunochromatography and application of test paper

A technology of immunochromatography and quantitative detection, which is applied in the field of medical detection, can solve problems such as difficulty in accurate detection and interference of red blood cell detection, and achieves the effects of easy production, wide linear detection range, and easy promotion

Active Publication Date: 2019-05-24
湖南达道生物工程有限公司
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  • Abstract
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Problems solved by technology

Because the amount of blood sampled from peripheral blood is usually less, it is difficult to meet the minimum sample size required by PCT or NT-proBNP level test strips in the prior art. There will be greater interference in the detection, and the existence of these problems makes it difficult to accurately detect the true content of markers such as PCT or NT-proBNP in the peripheral blood using the detection test strips in the prior art

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  • Test paper applicable to quantitative detection of peripheral blood immunochromatography and application of test paper

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Embodiment Construction

[0028] In order to describe the technical content, achieved goals and effects of the present invention in detail, the following descriptions will be made in conjunction with the embodiments and accompanying drawings.

[0029] Embodiment 1 of the present invention is: a test paper suitable for quantitative detection of peripheral blood immune chromatography, such as figure 1 As shown, it includes a base plate 1 and an upper cover (not shown in the figure), the upper cover is covered on the base plate 1, and the base plate 1 is sequentially provided with a sample pad 2, a sealing pad 3, Reaction pad 4 and water-absorbing pad 5; The sample pad 2 is coated with a first antibody, the first antibody has a tracer marker and can recognize an antigenic epitope of the target to be tested; the blocking pad 3 RBC is coated on it, and the sealing pad 3 and the reaction pad 4 are partially laminated. The upper cover is provided with a sample injection hole and an observation window in the ...

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Abstract

The invention discloses test paper applicable to quantitative detection of peripheral blood immunochromatography and application of the test paper in preparation of immunochromatography test kits or the quantitative detection of the peripheral blood immunochromatography. The test paper comprises a bottom plate, wherein a sampling pad, a sealing pad, a reaction pad and a water absorbing pad are sequentially arranged on the length direction of the bottom plate. The test paper is characterized in that the sample pad is coated with a first antibody, and the first antibody having a tracer marker can recognize an antigenic epitope of a target to be tested; the sealing pad is coated with an anti-erythrocyte antibody, and partially overlaps the reaction pad. Compared with the prior art, the test paper has the advantages of high sensitivity, wide linear range and the like.

Description

technical field [0001] The invention relates to the technical field of medical detection, in particular to a test paper suitable for quantitative detection of peripheral blood immune chromatography and its application. Background technique [0002] Immu-nochromatography assay (ICA) is a new type of immunoassay that appeared in the early 1980s. It is a simple and rapid immunological detection technology based on immunodiafiltration. The principle of immunochromatography is to immobilize specific antibodies on a certain zone of the nitrocellulose membrane. When one end of the dry nitrocellulose is immersed in the sample, the sample will move forward along the membrane due to the action of the capillary. When moving to the area where the antibody is immobilized, the corresponding antigen in the sample will specifically bind to the antibody. If stained with immunocolloidal gold or immunoenzyme, the area can display a certain color, thereby achieving specific immunodiagnosis. Th...

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Application Information

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IPC IPC(8): G01N33/558G01N33/543G01N33/58
Inventor 张雯娜邓朝义胡今科
Owner 湖南达道生物工程有限公司
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