Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Polyvalent pneumococci conjugate vaccine

A technology of pneumococcal and conjugated vaccines, applied in the field of multivalent pneumococcal conjugated vaccines, can solve problems such as difficult treatment

Inactive Publication Date: 2019-05-21
BEIJING ZHIFEI LVZHU BIOPHARM +2
View PDF20 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the published literature, there is no report on the preparation of conjugates of these two serotypes, but these two serotypes are currently the serotypes that cause the fastest rise in disease incidence in the world, especially these two serotypes are usually resistant , making it difficult to treat

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Polyvalent pneumococci conjugate vaccine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1 Preparation of pneumococcal 24F conjugate:

[0027] Dissolve the capsular polysaccharide of pneumococcus 24F with normal saline to 10-20 mg / ml, adjust the pH value to about 9.0, add CDAP at a mass ratio of 1:0.75, keep the pH value at 9.00 and react for 5 minutes, and add 2% in equal volume with CDAP Triethylamine solution, the pH value is 10.00-11.00, react for 5 minutes, adjust the pH value to 8.60, add the carrier protein TT according to the polysaccharide protein mass ratio 1:1, keep the pH value 8.6 and react for more than 120 minutes, glycine terminates the reaction, after ultrafiltration Obtain polysaccharide conjugates. The conjugate was purified by Sepharose 4FF chromatography, and the V 0 Nearby eluting peaks yield pneumococcal 24F conjugates.

Embodiment 2

[0028] Embodiment 2, pneumococcus 35B conjugate

[0029] Dissolve the capsular polysaccharide of pneumococcus 35B in water for injection to 10-20 mg / ml, adjust the pH value to about 9.0, add CDAP at a mass ratio of 1:0.75, keep the pH value at 9.00 and react for 5 minutes, and add 2% in equal volume with CDAP Triethylamine solution, the pH value is 10.00-11.00, react for 5 minutes, adjust the pH value to 8.60, add the carrier protein TT according to the polysaccharide protein mass ratio 1:0.5, maintain the pH value 8.6 and react for more than 120 minutes, glycine terminates the reaction, after ultrafiltration Obtain polysaccharide conjugates. The conjugate was purified by Sepharose 4FF chromatography, and the V 0 Nearby eluting peaks yield pneumococcal 35B conjugates.

Embodiment 3

[0030] Embodiment 3, other types of pneumococcal conjugates

[0031] Pneumococcus 1, 3, 4, 5, 6A, 6B, 8, 9A, 9N, 9V, 10A, 11A, 14, 15B, 17F, 18C, 19F, 19A, 20, 23F, 33F were prepared by the method of Example 1 ; Pneumococcus 2, 7F, 12F, 12A, 22F, 45, 46 were prepared by the method of Example 2.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a serotype combination of a polyvalent pneumococci conjugate vaccine. Pneumococci serotypes 24 and 35 (24F and 35B) contained in the serotype combination of the polyvalent pneumococci conjugate vaccine are used for preventing the infection caused by the pneumococci which contains the serotypes.

Description

technical field [0001] The present invention relates to a multivalent pneumococcal conjugate vaccine, which at least contains pneumococcus serotype 24 and serotype 35, and is used for preventing infection caused by the included serotypes of pneumococcus. Background technique [0002] Pneumococcus (Streptococcus pneumoniae) is the main pathogenic bacteria that causes pneumonia, meningitis, bacteremia, etc. in children and the elderly, and has a high morbidity and mortality rate in the world. At present, preventive preparations (vaccine) have been used in different populations, including pneumococcal polysaccharide vaccine used in people over 2 years old and pneumococcal conjugate vaccine used in infants and young children. There is only one type of pneumococcal polysaccharide vaccine, which is a 23-valent pneumococcal polysaccharide vaccine, including pneumonia serotypes 1, 2, 3, 4, 5, 6B, 7F, 8, 9N, 9V, 10A, 11A, 12F, 14, 15B, 17F, 18C, 19F, 19A, 20, 22F, 23F, and 33F. Pne...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A61K39/116A61K39/385A61K39/09A61K39/39A61P31/04
Inventor 杜琳杜真晖蒋凌峰
Owner BEIJING ZHIFEI LVZHU BIOPHARM
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com