Method for improving detection limit of Staphylococcus aureus in easily foamable liquid food
A staphylococcus, liquid food technology, applied in the field of detection, can solve the problems of foaming, increase the sampling volume, etc., to achieve the effect of avoiding missed detection, increasing the sampling volume, and increasing the detection rate
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[0027] A method for improving the detection limit of Staphylococcus aureus in easy-to-foam liquid food, comprising the following steps:
[0028] 1) Take 500mL of liquid food samples that are prone to foaming and add 10mL of polydimethylsiloxane (PMX) to defoam, centrifuge at 8000rmp / min for 6min under sterile conditions at 20°C, and collect the supernatant;
[0029] 2) The obtained supernatant is suction-filtered with a Φ50mm 0.45μm polyether PES microporous membrane, and the microorganisms are enriched on the microporous membrane;
[0030] 3) Put the microporous filter membrane in a Φ55mm 50mL flat-bottomed glass with a stopper, wash and soak it with 10mL Hanks eluent, and shake it on a micro-oscillator for 2 minutes to release the microorganisms in the micropores into the solution to obtain a sample concentrate; this operation requires Complete within 3 minutes;
[0031] 4) Detection of Staphylococcus aureus in samples
[0032] Mix 1mL sample concentrate with 9mL phosphate...
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