Centrifugal microfluidic chip and nucleic acid analysis system

Abstract

The invention relates to a centrifugal microfluidic chip and a nucleic acid analysis system, wherein the centrifugal microfluidic chip comprises a chip substrate, a sample adding cavity, an air outlet, a sample enrichment cracking cavity, a first waste liquid cavity, a first capillary valve, a dilution cavity, a second capillary valve, a second waste liquid cavity and a plurality of PCR amplification cavities. According to the centrifugal microfluidic chip and the nucleic acid analysis system, through the ingeniously designed cavity structure, the PCR amplification technology can be applied tothe centrifugal microfluidic technology to achieve the molecular diagnosis based on the PCR amplification, and through the sequential control of the liquid in the sample adding cavity, the sample enrichment cracking cavity, the dilution cavity and the PCR amplification cavity, the PCR amplification is achieved in the PCR amplification cavity. The whole reaction process is in a closed microfluidicchip structure, so the burden of operators and the possibility of pollution are reduced, the whole molecular diagnosis process does not depend on a molecular diagnosis laboratory or on professional operators any more, thereby meeting the requirement of quick detection anytime and anywhere, and bringing great help for medical examinations and disease prevention and control.

Description

technical field [0001] The present application relates to the field of centrifugal microfluidics, in particular to a centrifugal microfluidic chip and a nucleic acid analysis system. Background technique [0002] Microfluidics refers to the manipulation of liquids on the submillimeter scale, where the submillimeter scale is generally several microns to hundreds of microns. Microfluidic technology integrates the basic operating units involved in the fields of biology and chemistry, and even the functions of the entire laboratory, including sampling, dilution, reaction, separation, detection, etc., on a small chip, so it is also called lab-on-a-chip ( Lab-on-a-Chip). This kind of chip is generally composed of various liquid storage pools and interconnected microchannel networks, which can greatly shorten the sample processing time, and realize the maximum utilization efficiency of reagent consumables through precise control of liquid flow. [0003] Microfluidic systems are d...

AI Technical Summary

Problems solved by technology

[0005] However, in the molecular diagnostic system based on PCR amplification, due to aerosol pollution during PCR amplification, and in order to avoid cross-contamination between samples, it is generally necessary to set up a partitioned laboratory

This laboratory needs to implement sample processing, nucleic acid extraction, and PCR amplification partition operations, and must have a good ventilation system. The cost of laboratory construction is high, and often only large medical institutions have the financial resources to build it.

On the other hand, laboratory operators have to hold certificates to work, which also greatly increases labor costs

At the same time, too much manual intervention will inevitably lead to human error

All of these have greatly raised the technical threshold of PCR-based molecular diagnosis

[0006] Moreover, in the current molecular diagnostic laboratory mode, multi-sample and multi-test project operations are completed in a centralized experimental site, and the process quality control requirements are high.

Moreover, the current molecular diagnostic laboratory mode is generally a multi-sample single-indicator detection mode, and the detection indicators are limited, so it is impossible to screen single-sample multi-indicator infectious pathogens

In addition, although the advantages of molecular diagnostic technology are obvious, molecular diagnostics are also expensive due to the cumbersome steps, time-consuming process, and the need for professionals to operate, and the construction cost of clinical molecular diagnostic laboratories is generally high.

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