Extraction method of natural bamboo juice polysaccharides
An extraction method, bamboo juice technology, applied in the field of extraction of natural bamboo juice polysaccharides, to achieve the effect of improving purity, high purity, and avoiding yield reduction
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Embodiment 1
[0023] A method for extracting natural bamboo juice polysaccharides, comprising the steps of:
[0024] (1) Juice extraction: From 20:00 every night to 06:00 the next morning, from the green bamboo in Guangxi [Taiwan Sweet Bamboo, Dendrocalamopsis oldhami (Munro) Keng f.] from natural bamboo juice.
[0025] (2) Impurity removal and preservation: quickly filter the obtained bamboo juice to remove insoluble impurities, then put the obtained bamboo juice into a sterilized food-grade plastic container, and place it in a refrigerator at 0-4°C for later use.
[0026] (3) Concentration at low temperature: Put 2000mL of bamboo juice in a rotary evaporator, and concentrate it to 50mL under a negative pressure of 0.1MPa and a low temperature of 50°C to obtain a concentrated solution.
[0027] (4) Ethanol precipitation: Mix the concentrated solution and ethanol evenly at a volume ratio of 1:8, then let it stand for 2 hours to settle, wait for the precipitation to separate out, and carry...
Embodiment 2
[0036] A method for extracting natural bamboo juice polysaccharides, comprising the steps of:
[0037] (1) Juice extraction: From 20:00 every night to 06:00 the next morning, from the green bamboo in Guangxi [Taiwan Sweet Bamboo, Dendrocalamopsis oldhami (Munro) Keng f.] from natural bamboo juice.
[0038] (2) Impurity removal and preservation: quickly filter the obtained bamboo juice to remove insoluble impurities, then put the obtained bamboo juice into a sterilized food-grade plastic container, and place it in a refrigerator at 0-4°C for later use.
[0039] (3) Concentration at low temperature: put the bamboo juice in a rotary evaporator, and concentrate it at a negative pressure of 0.15MPa and a low temperature of 40°C to obtain a 40-fold concentrated solution.
[0040] (4) Ethanol precipitation: Mix the concentrated solution and ethanol evenly at a volume ratio of 1:8, and then set aside to settle for 2 hours. After the precipitation is separated, perform centrifugation, ...
Embodiment 3
[0049] A method for extracting natural bamboo juice polysaccharides, comprising the steps of:
[0050] (1) Juice extraction: From 20:00 every night to 06:00 the next morning, from the green bamboo in Guangxi [Taiwan Sweet Bamboo, Dendrocalamopsis oldhami (Munro) Keng f.] from natural bamboo juice.
[0051] (2) Impurity removal and preservation: quickly filter the obtained bamboo juice to remove insoluble impurities, then put the obtained bamboo juice into a sterilized food-grade plastic container, and place it in a refrigerator at 0-4°C for later use.
[0052] (3) Concentration at low temperature: put the bamboo juice in a rotary evaporator, and concentrate it at a negative pressure of 0.05MPa and a low temperature of 60°C to obtain a 40-fold concentrated solution.
[0053] (4) Ethanol precipitation: mix the concentrated solution and ethanol evenly at a volume ratio of 1:8, and then set aside to settle for 3 hours. After the precipitation is separated, carry out centrifugati...
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