Individual genetic test reagent kit of medicines for relieving fever and alleviating pain

A gene detection, antipyretic and analgesic technology, applied in the biological field, can solve the problems affecting the treatment effect, toxic and side effects, etc.

Pending Publication Date: 2019-05-03
山东中科翰康医学检验有限公司 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, antipyretic and analgesic drugs in clinical use have antipyretic, analgesic, anti-inflammatory and anti-rheumatic effects, and there are many types, such as aminopyrine, phenylbutazone, ibuprofen, paracetamol and compound preparation ant

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Prepare a kit for detecting the metabolism of antipyretic and analgesic drugs for one person, including the following components:

[0030] (1) 4 pairs of CYP2D6*10, CYP2C9*3, CYP2C19*2 / *3 gene PCR amplification and sequencing primers, 1 OD for each primer, see Table 1 for primer sequences;

[0031] (2) PCR amplification reagent: 7.5 µL of Taq enzyme mixture (dNTP, l0× PCR reaction buffer, MgCl 2 );

[0032] (3) PCR product purification reagent: 1.6 µL SAP enzyme mixture (SAP enzyme, ExoI enzyme, deionized water);

[0033] (3) Sequencing reagents: 2.2 µL BigDye mix (Bigdye, 5×seq), 2.5 µL EDTA, 30 µL ethanol solution (100%), 100 µL ethanol solution (70%), 8 µL Hi-Di.

[0034] This kit is stored at -20°C, and repeated freezing and thawing should be avoided as much as possible.

Embodiment 2

[0036] The steps to use the kit for detecting the metabolic capacity of antipyretic and analgesic drugs for one person include:

[0037] (1) Extract the genomic DNA of the sample;

[0038] (2) PCR amplification of CYP2D6*10, CYP2C9*3, CYP2C19*2 / *3 genes

[0039] Design primers for CYP2D6*10, CYP2C9*3, and CYP2C19*2 / *3 genes according to Table 2 for PCR amplification. Each reaction system has a total volume of 50ul, including 25µL of 2*Mix mixture, 17µL of deionized water, upstream and downstream 3 µL of each primer, 2 ul of genomic DNA; the PCR reaction conditions were 95°C for 3 minutes for pre-denaturation, followed by 35 cycles of 95°C for 30 sec, 60°C for 20 sec, and 72°C for 30 sec; and finally extension at 72°C for 5 minutes;

[0040] (3) Purification of PCR products

[0041] Each reaction system has a total volume of 5.6 µL, 4 µL of PCR product, and 1.6 µL of SAP enzyme mixture (SAP enzyme, ExoI enzyme, deionized water). The reaction conditions are 37°C for 45 minute...

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PUM

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Abstract

The invention belongs to the field of biological technology, relates to a genetic test reagent kit, in particular to an individual genetic test reagent kit of medicines for relieving fever and alleviating pain. The reagent kit comprises the main components of: (1) 4 pairs of CYP2D6*10, CYP2C9*3 and CYP2C19*2/*3 gene PCR amplification and sequencing primers; (2) preparing a PCR amplification reagent; (3) preparing a PCR product purifying reagent; and (4) preparing a DNA sequencing reagent. The reagent kit can be used for detecting the metabolic capability of the medicines for relieving fever and alleviating pain, and understanding the metabolic capability of the medicines in bodies of children, can effectively and objectively assess the treatment effect and adverse reactions of the medicines to the children, and is favorable for reduction of damage of the medicines to the children, so that the treatment effect is maximized.

Description

technical field [0001] The invention belongs to the field of biological technology and relates to a gene detection kit, in particular to a kit for personalized gene detection based on antipyretic and analgesic drugs. Background technique [0002] It has been reported that children are in the period of growth and development, the nervous system, endocrine system and many organs are not fully developed, their physiological functions are immature, and their detoxification function is also poor. The absorption, distribution, metabolism and excretion of drugs are different from those of adults There are significant differences; if the medication is inappropriate, it is more likely to accumulate in the body than adults, directly damage the liver and kidney, and in severe cases, it will cause liver and kidney failure, and it will also cause other diseases, cause disability, and even endanger life. Some studies have found that the side effects of some drugs may not be obvious at the...

Claims

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Application Information

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IPC IPC(8): C12Q1/6869
Inventor 马端姜昕徐沁赵振峰徐海龙景丹丹王斐
Owner 山东中科翰康医学检验有限公司
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