Preparation method of electrochemical sensor for detecting zearalenone in grain or feed

A zearalenone, electrochemical technology, applied in the direction of material electrochemical variables, scientific instruments, instruments, etc., can solve the problems of complexity and difficulty in ensuring accuracy, and achieve the goal of ensuring specificity, facilitating commercialization, and promoting development Effect

Inactive Publication Date: 2019-04-30
CHONGQING MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although these methods can accurately detect mycotoxins in food or feed samples, they require skilled operators, complicated sample pretreatment and expensive equipment, and it is difficult to ensure accuracy at trace levels

Method used

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  • Preparation method of electrochemical sensor for detecting zearalenone in grain or feed
  • Preparation method of electrochemical sensor for detecting zearalenone in grain or feed
  • Preparation method of electrochemical sensor for detecting zearalenone in grain or feed

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Embodiment 1

[0043] Step 1. Add 31.66 mg of citric acid monohydrate to 35 mL of ultrapure water. Keep the solution at 80 °C and add 170 μl of 5% HAuCl 4 . When the color of the mixture changed from pale yellow to pink, 100 μl of aniline was added. Subsequently, after reacting at 80°C for 2 minutes, 125 µl of 5% HAuCl4 was added to the solution, followed by heating for 5 minutes. The mixture solution was kept at room temperature for 2 hours. Finally, the obtained gold-polyaniline-gold (Au-PANI-Au) was purified by centrifugation and washed with ultrapure water;

[0044] Step 2. Use 0.3 and 0.05 μm Al respectively 2 o 3 Polish the electrode into a mirror surface with powder, then ultrasonically ultrasonicate the electrode for 5 minutes each in the order of ultrapure water, absolute ethanol, and ultrapure water, and dry at room temperature for later use;

[0045] Step 3. Add 10 μL of the electrode modification material gold-polyaniline-gold (Au-PANI-Au, 2.4 mg / mL) dropwise on the electro...

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Abstract

The invention relates to a preparation method and application of an electrochemical sensor for mycotoxin zearalenone detection, and belongs to the technical field of electrochemical detection. The preparation method is characterized by comprising the following steps: firstly, synthesizing to obtain a Cu at L-Glu nano-material, then immobilizing palladium-platinum bimetallic nanoparticles on the Cuat L-Glu nano-material, and mixing a signal probe with the composite material to prepare a biological signal probe; and immobilizing a zearalenone aptamer AP by using Au-PANI-Au so as to prepare theelectrochemical sensor for zearalenone detection. The sensor has the potential for zearalenone detection in food and feed. The method has the advantages of high sensitivity, high specificity and rapidness and convenience in detection. Experimental basis is provided for research and development of zearalenone pollution detection technology, and a new idea and a new technical platform are provided for monitoring zearalenone pollution in food and feed.

Description

Technical field: [0001] The invention relates to a preparation method and application of an electrochemical sensor for quantitative detection of zearalenone in grains, especially a biosensor based on copper organic coordination polymers and bimetallic palladium-platinum nanocomposites as signal probes. The specific recognition function of the nucleic acid aptamer to the zearalenone is used to detect the zearalenone, which belongs to the field of electrochemical detection. Background technique: [0002] Diseases caused by food-borne hazards are important factors that endanger public health in both developed and developing countries, and the hazards of food contamination to residents' health have attracted widespread attention from governments and the international community. Zearalenone is mainly a mycotoxin produced by Fusarium graminearum, and it is one of the most widely polluted Fusarium toxins in grains. Zearalenone can be detected in grains and agricultural by-products....

Claims

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Application Information

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IPC IPC(8): G01N27/26G01N27/30G01N27/327
CPCG01N27/26G01N27/30G01N27/3278
Inventor 何俊琳于超吉星多
Owner CHONGQING MEDICAL UNIVERSITY
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