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A method for measuring microcystin concentration

A microcystin and concentration technology, applied in the field of environmental protection, can solve the problems of low selectivity, high cost, and complicated operation of the detection method, and achieve the effect of simple operation, low detection limit, and avoiding background fluorescence interference

Active Publication Date: 2021-07-27
YANGTZE NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In view of the above-mentioned deficiencies in the prior art, the object of the present invention is to provide a method for determining the concentration of microcystins, which solves the problems of complex operation, high cost and low selectivity of the existing detection methods.

Method used

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  • A method for measuring microcystin concentration
  • A method for measuring microcystin concentration
  • A method for measuring microcystin concentration

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] 1) Preparation of molybdenum disulfide quantum dot stock solution with upconversion fluorescence:

[0038] Ammonium molybdate tetrahydrate, N-acetyl-L-cysteine ​​and thiourea were weighed according to the mass ratio of 3.16:1:0.39, and they were added to water and fully mixed under an ice bath to obtain a mixed solution, wherein, For each gram of sodium molybdate tetrahydrate, 64.7 mL of water needs to be added, and then the mixed solution is transferred to a hydrothermal reaction kettle for 4 hours at 200°C, cooled naturally, and then subjected to a centrifuge with a speed of 20,000 r / min. After centrifugation for 10 min, the supernatant was taken to obtain the original solution of molybdenum disulfide quantum dots.

[0039] 2) Draw a standard curve:

[0040] Take the microcystin nucleic acid aptamer solution with a concentration of 2 μM, the gold nanometer solution reduced with sodium borohydride with a concentration of 14.4 nM, the NaCl solution with a concentration...

Embodiment 2

[0048] 1) Preparation of molybdenum disulfide quantum dot stock solution with upconversion fluorescence:

[0049] Ammonium molybdate tetrahydrate, N-acetyl-L-cysteine ​​and thiourea were weighed according to the mass ratio of 3.16:1:0.39, and they were added to water and fully mixed under an ice bath to obtain a mixed solution, wherein, For each gram of sodium molybdate tetrahydrate, 64.7 mL of water needs to be added, and then the mixed solution is transferred to a hydrothermal reaction kettle for 4 hours at 220°C, cooled naturally, and then subjected to a centrifuge with a speed of 20,000 r / min. After centrifugation for 10 min, the supernatant was taken to obtain the original solution of molybdenum disulfide quantum dots.

[0050] 2) Draw a standard curve:

[0051] Take 5 μL of 2 μM microcystin nucleic acid aptamer solution, 100 μL of 14.4 nM sodium borohydride-reduced gold nano-solution and 30 μL of 100 mM KCl solution to obtain a mixed solution, and then add to the above ...

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Abstract

The present invention provides a method for determining the concentration of microcystins, based on the fact that molybdenum disulfide quantum dots can interact with gold nanoparticles through the inner filter effect, but microcystins can affect the aptamer-modified gold nanoparticles in The degree of aggregation and dispersion in high-salt solution realizes the detection of microcystin concentration in water environment. The up-conversion fluorescence of molybdenum disulfide used in the present invention can effectively avoid the interference of background matrix fluorescence in the water environment, has good optical stability, accurate and reliable detection results, low cost, simple operation, environmental protection, and good detection specificity. Avoid the influence of other common metal ions or anions on the determination of microcystins, strong anti-interference ability, sensitive and reliable results, low detection limit, fast response speed, the whole reaction process only takes 20 minutes, which greatly improves the detection efficiency and is stable It has good performance, can realize rapid and specific detection on-line in real time, and can be used for simple and rapid detection of microcystins in actual water environment.

Description

technical field [0001] The invention belongs to the technical field of environmental protection, and in particular relates to a method for measuring the concentration of microcystins based on up-conversion fluorescence molybdenum disulfide quantum dots. Background technique [0002] Microcystins (MCs) are a class of cyclic heptapeptide hepatotoxins released by harmful cyanobacteria blooms. They have strong carcinogenic effects and are important environmental factors that induce liver cancer, gastroenteritis and other diseases. In view of the toxicity and harm of MCs, the World Health Organization (WHO) has listed MCs as harmful pollutants to be controlled in drinking water. MCs are listed together with hepatitis virus and aflatoxin as the three major environmental risk factors that induce the high incidence of primary liver cancer in southern my country. In this regard, the newly promulgated "Hygienic Standards for Drinking Water" (GB5749-2006) in 2006 The content of MCs is ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/64
CPCG01N21/6428G01N2021/6432
Inventor 曹海燕石文兵董文飞陈果
Owner YANGTZE NORMAL UNIVERSITY
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