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Method for detecting biomass and composition concentrations in fermentation process based on near-infrared spectrum

A technology of near-infrared spectroscopy and fermentation process, which is applied in the field of industrial process detection

Inactive Publication Date: 2019-04-23
DALIAN UNIV OF TECH
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Problems solved by technology

[0005] The technical problem to be solved by the present invention is: for the simultaneous measurement of biomass, substrate concentration and product concentration in the biological fermentation process, the traditional method is to use near-infrared spectroscopy to establish detection models for different components, but for multiple groups Establishing a joint calibration model separately for detection has better accuracy and convenience

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  • Method for detecting biomass and composition concentrations in fermentation process based on near-infrared spectrum
  • Method for detecting biomass and composition concentrations in fermentation process based on near-infrared spectrum
  • Method for detecting biomass and composition concentrations in fermentation process based on near-infrared spectrum

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Embodiment Construction

[0047] The specific implementation manners of the present invention will be further described below in conjunction with the accompanying drawings and technical solutions.

[0048] The embodiment is the online detection of biomass, glucose concentration and ethanol concentration in batch ethanol fermentation process. The established online monitoring experimental platform for the ethanol fermentation process such as figure 2 shown. The capacity of the fermentation tank is 2.5L, and the temperature, pH value, and rotating speed of the stirring paddle in the tank are controlled by the fermentation control equipment. Use a platinum thermometer PT100 to measure the temperature in the fermenter, and control the temperature through a heating device and circulating cooling water. The pH of the fermentation broth is adjusted by NaOH solution, and the pH value in the fermenter is detected by a pH meter in real time; the solution in the fermenter is evenly stirred by an electric stirr...

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Abstract

The invention belongs to the field of industrial process detection and relates to a method for detecting biomass and composition concentrations in a fermentation process based on a near-infrared spectrum. The method is characterized by building an on-line monitoring experiment platform of a biological fermentation process by using a near-infrared spectrum analyzer equipped with an immersion probe,so as to carry out real-time in-situ measurement of near-infrared spectrum data of fermentation liquid of the biological fermentation process. The method comprises the following steps: firstly, collecting spectrum data and reference data; secondly, carrying out preprocessing operation on the measured near-infrared spectrum data, then dividing the data into a correction set and a validation set and building a joint calibration model, and selecting model parameters by using a grid searching and cross validation method; finally, validating the validity of the established model through external experiment, so as to carry out quantitative analysis of biomass, a substrate concentration and a product concentration of the fermentation process. The method is capable of automatically and rapidly detecting the biomass, the substrate concentration and the product concentration; practical industrial application and popularization of the method are facilitated.

Description

technical field [0001] The invention belongs to the field of industrial process detection, and relates to a method for detecting biomass and component concentrations in a fermentation process based on near-infrared spectroscopy, specifically a method for real-time measurement of biomass, substrate concentration and product concentration in a fermentation process based on near-infrared spectroscopy analysis technology . Background technique [0002] Fermentation engineering is widely used in biomass conversion, food, medicine and other production industries. The parameters of the fermentation process are mainly divided into chemical parameters (such as pH value, dissolved oxygen concentration, etc.), physical parameters (such as fermentation temperature, stirring speed, etc.) and biological parameters (substrate concentration, biomass concentration and product concentration, etc.). In engineering applications, the fermentation process is mainly controlled and optimized by ad...

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Application Information

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IPC IPC(8): G01N21/359G01N21/3577
CPCG01N21/3577G01N21/359
Inventor 刘涛王旭东孙旭东薛闯孟庆伟仲崇权
Owner DALIAN UNIV OF TECH
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