Multi-index combined detection marker protein paper chip

Abstract

The invention relates to a multi-index combined detection marker protein paper chip, and belongs to the technical field of disease detection. The paper chip comprises two parts including a detection channel region and a water absorption region. The detection channel region is divided into hydrophilic detection channels between adjacent wax areas through wax spraying and printing of the rectangularwax areas on a nitrocellulose membrane. The detection channel is divided into a sample region, a reaction region and a color developing region, and is a place for reaction detection. The water absorption region is partially overlapped with a detection region through water absorption filter paper, provides chromatography power and absorbs excessive reactants. Maker protein in a sample reaches thereaction region through chromatography, and is combined with a specific antibody coated with colloidal gold; the color developing region is used for presenting a detection result in a red strip visible to naked eyes so that the paper chip can be suitable for daily family diagnosis. The paper chip has high accuracy and provides a simple and fast tool for joint detection of disease-related marker protein.

Description

technical field [0001] The invention belongs to the field of disease detection, is based on colloidal gold technology and wax spray printing to prepare microfluidic paper chip technology, and is designed as a household diagnostic device for joint detection of two or more marker proteins. Background technique [0002] For the detection of landmark protein indicators such as tumor markers, traditional methods such as enzyme-linked immunosorbent assay, the detection steps are cumbersome and time-consuming; only one indicator can be detected each time, resulting in low specificity and low false positive rate. High; the final display of the test results requires certain analytical equipment, which limits its applicable places. In response to these shortcomings, the present invention proposes a multi-index joint detection paper microfluidic chip (referred to as paper chip) combined with wax spray printing to prepare a microfluidic paper chip channel and a colloidal gold-labeled sp...

AI Technical Summary

Problems solved by technology

[0002] For the detection of landmark protein indicators such as tumor markers, traditional methods such as enzyme-linked immunosorbent assay, the detection steps are cumbersome and time-consuming; only one indicator can be detected each time, resulting in low specificity and low false positive rate. High; the final display of the test results requires certain analytical equipment, which limits its applicable places

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