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Nucleic acid reagents, kits, systems and methods for detecting respiratory tract infection pathogens

A technology for respiratory tract and pathogens, applied in the field of nucleic acid reagents for detecting respiratory tract infection pathogens, can solve the problems of complicated operation, time-consuming, low sensitivity, etc., and achieve the effects of simple detection process, automatic result determination, and reliable automatic interpretation.

Active Publication Date: 2019-09-13
北京卓诚惠生生物科技股份有限公司
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Problems solved by technology

However, there are common problems in virus isolation and neutralization experiments: cumbersome operation, time-consuming, low sensitivity, not suitable for early rapid on-site diagnosis
It should be noted that the extraction of nucleic acid in the above two invention patent applications is achieved through manual operation, and the judgment of the result is manually judged through the instructions, which has a certain degree of subjectivity
[0007] Commonly used PCR detection requires complex sample processing, cumbersome test procedures and complex result judgments
In addition, the detection targets that RT-PCR can cover are limited, and multi-system settings are required to achieve multi-target detection, which increases the complexity of the operation

Method used

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  • Nucleic acid reagents, kits, systems and methods for detecting respiratory tract infection pathogens
  • Nucleic acid reagents, kits, systems and methods for detecting respiratory tract infection pathogens
  • Nucleic acid reagents, kits, systems and methods for detecting respiratory tract infection pathogens

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Embodiment

[0072] 1. Primer and probe synthesis

[0073] Sequence synthesis was performed according to the primer sequences shown in Table 1 and the probe sequences shown in Table 2. In the sequence, Y represents the degenerate base T / C; R represents the degenerate base A / G. In the probe, FAM is 6-carboxyfluorescein, JOE is 2,7-dimethyl-4,5-dichloro-6-carboxyfluorescein, CY5 is 5H-indocyanine, and ROX is 6-carboxy-X-rhodan bright. The brackets in the probe sequences in Table 2 indicate that the t on the left side of the brackets has a fluorescent label, and the contents in the brackets indicate the choice of fluorescent labels.

[0074] Table 1

[0075]

[0076]

[0077] Table 2

[0078] detection target probe code probe sequence Tm value (℃) SEQ ID NO InfluA fluA-P aggccccct(fam)caaagccgagat(fam)cgcgcaga 69 27 InfluB fluB-P ccaatt(joe)cgagcagct(joe)gaaactg 64 28 ADV ADV-P acacct(fam)acttcagt(fam)atgg 60 29 HBoV HBoV-P ...

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Abstract

The present disclosure relates to a nucleic acid reagent, kit, system and method for detecting a respiratory infection pathogen. The nucleic acid reagent comprises primers shown as SEQ ID NO.1-24 andprobes shown as SEQ ID NO.27-45, wherein the primers and probes are stored separately from each other or arbitrarily mixed with each other. The present establishes the nucleic acid reagent, kits, system and method for detecting the respiratory infection pathogen by the primers and probes described above, and can realize rapid, comprehensive, sensitive, specific and automatic detection result determination.

Description

technical field [0001] The present disclosure relates to the field of biotechnology, in particular, to a nucleic acid reagent, kit, system and method for detecting pathogens of respiratory tract infection. Background technique [0002] Respiratory tract infection (Respiratory tract infection, RTI) is the most common type of human disease, widely occurring in any age, gender and region, causing serious disease burden to individuals, families and society. Viral infection is an important cause of respiratory tract infection. In developed countries, acute viral respiratory tract infection is the leading cause of hospitalization of infants and children; in developing countries, acute viral respiratory tract infection is the main cause of death. In addition to viruses, Mycoplasma pneumoniae and Chlamydia pneumoniae, which are between viruses and bacteria, often cause atypical pneumonia, causing symptoms other than respiratory tract infections, and are easy to cause epidemics in de...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/70C12Q1/689C12Q1/04C12Q1/686C12N15/11C12M1/34
CPCC12Q1/686C12Q1/689C12Q1/701C12Q2600/16C12Q2600/166C12Q2537/143C12Q2563/107C12Q2527/107C12Q2545/101Y02A50/30
Inventor 张志强
Owner 北京卓诚惠生生物科技股份有限公司
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