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Electrochemical immunosensor and preparation method and application thereof

An immunosensor, electrochemical technology, applied in the direction of electrochemical variables of materials, etc., can solve the problems that the stability of electrode modification materials needs to be improved, the specificity of single antibody-antigen reaction needs to be enhanced, etc., to achieve effective and convenient evaluation, reduce trauma and infection. , the effect of reducing cancer mortality

Active Publication Date: 2019-04-09
JIAXING UNIV
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AI Technical Summary

Problems solved by technology

The PSA electrochemical immunosensor based on these materials has the advantages of high sensitivity, fast and high efficiency, but also has certain shortcomings, such as the stability of the electrode modification material needs to be improved or the specificity of the single antibody-antigen reaction needs to be enhanced.

Method used

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  • Electrochemical immunosensor and preparation method and application thereof
  • Electrochemical immunosensor and preparation method and application thereof
  • Electrochemical immunosensor and preparation method and application thereof

Examples

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Effect test

Embodiment 1

[0051] 1. Preparation of graphene oxide / polyionic liquid composite (GO-PIL)

[0052] Weigh 0.011g of graphene oxide, put it in a small beaker with 20mL of methanol, and ultrasonicate at room temperature for 30min, so that the graphene oxide is evenly dispersed in the methanol solution. Then take by weighing 0.058g AIBN and 0.022g ionic liquid, join in the methanol solution of the good graphene oxide of ultrasonic, N 2 Keep it in the environment for 40 minutes, seal it, heat and stir in an oil bath at 70°C for 14-15 hours, cool and centrifuge, wash and dry.

[0053] 2. Preparation of modified electrodes

[0054] Take 2 mg of GO-PIL dried in step 1 and disperse it in 1 mL of twice-distilled water, and mix it with 1.5% chitosan acetic acid solution 1:1 by ultrasonic to obtain a 1 mg / mL GO-PIL solution that can be modified for electrodes.

[0055] The platinum electrode was ground and polished to a mirror surface with aluminum oxide powder, and the surface of the electrode was r...

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Abstract

The invention discloses an electrochemical immunosensor and a preparation method thereof, and application in preparation of a PSA detection kit, and belongs to the technical field of electrochemical immunosensors. The electrochemical immunosensor comprises an operating electrode, the operating electrode comprises a modified electrode whose platinum electrode surface is wrapped by a graphene oxide / polyion liquid composite material layer, and an antibody 1 and an antibody 2 in specific binding with a to-be-analyzed target object, the antibody 1 is fixed to the graphene oxide / polyion liquid composite material layer, and the antibody 2 is marked with ferrocenecarboxylic acid. According to the electrochemical immunosensor, a novel graphene oxide / polyion liquid compound is provided and modifiedon the bare platinum electrode, a sandwich immunity mode is adopted to prepare the electrochemical immunosensor, the ferrocene-marked antibody 2 has a signal amplification effect, and the lowest detection limit of the sensor used for PSA detection reaches 1.0 pg / mL.

Description

technical field [0001] The invention relates to the technical field of electrochemical immunosensors, in particular to an electrochemical immunosensor, a preparation method thereof and an application in preparing a PSA detection kit. Background technique [0002] PSA (prostate specific antigen, prostate specific antigen) is a glycoprotein directly secreted by prostate epithelial cells into the prostate duct system. The PSA content in the serum of normal people is generally not higher than 4ng / mL, but when the prostate cancer occurs, the PSA content in the serum will rise sharply. Therefore, PSA has become a biomarker of prostate cancer, and it is also considered to be the most organ-specific marker among tumor markers. In recent years, the initial screening of PSA as the only marker of prostate cancer has been internationally recognized and has been clinically used for many years. In addition, the content of PSA in the serum can also reflect the stage of the patient's dise...

Claims

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Application Information

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IPC IPC(8): G01N27/48
CPCG01N27/48
Inventor 路义霞叶宝林曾延波尹争志王海龙李蕾
Owner JIAXING UNIV
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