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Methods of priming a sus' immune system

An immune system and animal technology, applied in the field of activating the immune system of pigs, can solve the problems of time-consuming, defective, and poor convenience in separating or extracting cell wall nuclear components

Inactive Publication Date: 2019-04-02
APTIMMUNE BIOLOGICS INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] An additional disadvantage is that it is inconvenient to administer only the core component of the cell wall
For example, isolating or extracting cell wall nuclear fractions can be time consuming and requires several steps
Yet another possible disadvantage is that the cell wall core components are not soluble in aqueous formulations and require lipid or oil based emulsions for delivery
[0011] While strategies are available to address the aforementioned issues (regarding respiratory infections that cause major mortality in piglets), these strategies can be inconvenient, flawed and less effective than other strategies

Method used

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  • Methods of priming a sus' immune system
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  • Methods of priming a sus' immune system

Examples

Experimental program
Comparison scheme
Effect test

Embodiment approach

[0060]1. A method for activating the immune system of an animal of the genus Suis, the method comprising administering an effective amount of whole cell lysate of mycobacteria to the animal of the genus Suis within an effective period after birth of the animal of the genus Suis.

[0061] 2. The method of clause 1, wherein the mycobacterial whole cell lysate is prepared from Mycobacterium smegmatis.

[0062] 3. The method of clause 1 or clause 2, wherein the mycobacterial whole cell lysate has not been purified.

[0063] 4. The method of any one of clauses 1 to 3, wherein the mycobacterial whole cell lysate is not fractionated.

[0064] 5. The method of any one of clauses 1 to 4, wherein the mycobacterial whole cell lysate is lipid-free.

[0065] 6. The method of any one of clauses 1 to 5, wherein the mycobacterial whole cell lysate is deproteinized.

[0066] 7. The method of any one of clauses 1 to 6, wherein the administering is selected from the group consisting of oral, i...

Embodiment 1

[0189] Significant production of TNF-α by porcine AMΦ stimulated with M. smegmatis WCL

[0190] The ability of ZMAC cells to produce TNF-α in response to LPS has been shown in previous studies. To test the immunostimulatory activity of M. smegmatis WCL, ZMAC cells were exposed to M. smegmatis WCL grown in 7H9 broth optimized for mycobacterial cultures. Cells were initially stimulated with a high concentration of 10 ug / mL M. smegmatis for 12 and 24 hours. Such as figure 1 As shown, the results demonstrate a lower production of TNF-α when porcine AMΦZMAC is exposed to the bacterial product lipopolysaccharide (LPS), which is a potent stimulator of TNF-α production, when porcine alveolar enlargement Burst production of tumor necrosis factor (TNF)-α by phagocytes (AMΦ) ZMAC exposed to M. smegmatis WCL.

Embodiment 2

[0192] Most TNF-α production by AMΦ in response to M. smegmatis WCL occurs within the first 6 hours after stimulation pregnancy

[0193] While data from previous experiments showed the production of significant amounts of TNF-[alpha] 12 hours post-stimulation, there appeared to be no further production of this cytokine during the 12-24 hour period. This finding led the inventors of the present disclosure to question whether the response of TNF-α to M. smegmatis WCL resembles the similar expression kinetics that have been observed for the response of this cytokine to LPS stimulation, which typically occurs within 4 days after stimulation. - peak within 6 hours. Therefore, a time analysis was set up to establish the kinetics of TNF-α production in response to stimulation of M. smegmatis WCL. In this experiment, the inventors of the present disclosure also included two other WCLs prepared from the same M. smegmatis but cultured in different broth types. Such as figure 2 A...

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Abstract

Methods of priming a Sus' immune system are disclosed. The methods comprise administering an effective amount of a Mycobacterial whole cell lysate to a Sus within an effective period of time after theSus is born.

Description

Background technique [0001] Respiratory infections are the leading cause of mortality in suckling pigs of nursing age ranging from about 19 days to about 68 days, leading to significant economic losses in the swine industry. 1 For example, porcine reproductive and respiratory syndrome (PRRS) is a global chronic viral disease of pigs. PRRS is endemic in most swine producing countries and is a major cause of economic loss to the swine industry, with annual losses estimated at $664 million in the United States. 2 The clinical signs of PRRS include respiratory and reproductive dysfunction, and the causative agent is PRRS virus ("PRRSV"). 3 PRRSV develops disease by overpowering the pig's immune system starting as early as two days and persisting for seven weeks after infection. 4 [0002] Vaccination of suckling pigs is a common means of combating respiratory infections; however, attempts to use the vaccination method to protect newborn piglets have been found to be ineffective...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/04A61K39/02A61K39/39A01K67/02A61P37/04
CPCA61K39/04A61K39/39A61K2039/552A01K67/027A61P37/04A61K2039/52A61K2039/543A61K2039/545A61P31/04A01K67/02A01K2227/108
Inventor 阿龙·吉尔贝蒂史蒂文·贝格尔费代里科·朱克曼
Owner APTIMMUNE BIOLOGICS INC
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