Mycotoxin biodegradation agent containing glucose oxidase and saccharomyces cerevisia and application of mycotoxin biodegradation agent

Abstract

The invention relates to a mycotoxin biodegradation agent containing glucose oxidase and saccharomyces cerevisia and an application of the mycotoxin biodegradation agent. The problems of production ofa mycotoxin biodegradation agent, degradation of aflatoxin B1, zearalenone, vomitoxin and T-2 toxin, and realization of the application of the mycotoxin biodegradation agent to mildew and rot feeds for piggies and broilers can be effectively solved. The mycotoxin biodegradation agent is prepared from glucose oxidase and saccharomyces cerevisia yk18 fermentation cultures in a mass ratio of the glucose oxidase to the saccharomyces cerevisia yk18 fermentation cultures being 1 to (1-9), wherein the glucose oxidase is a powdery feed additive namely glucose oxidase, the enzyme activity is greater than or equal to 2000 u / g and the enzyme activity is greater than or equal to 5000 u / g, or the glucose oxidase is a liquid feed additive namely glucose oxidase, and the enzyme activity is greater thanor equal to 10000 u / mL; and the saccharomyces cerevisia yk18 fermentation cultures are prepared through the steps of activating saccharomyces cerevisia yk18 on a flat seed culture medium, then inoculating 2% by v / v of the activated saccharomyces cerevisia yk18 in liquid fermentation mediums, performing culturing, then adding 5% by v / v of the liquid fermentation mediums to obtain a saccharomyces cerevisia yk18 liquid fermentation culture solution, then performing sealed culture with solid culture mediums, and performing air drying. The method is simple, the mycotoxin biodegradation agent is easy to produce and prepare, and the degradation agent is high in enzyme activity and is a creation in the mycotoxin biodegradation agent.

Description

technical field [0001] The invention relates to microorganisms, in particular to a mycotoxin biodegradation agent containing glucose oxidase and Saccharomyces cerevisiae and application thereof. Background technique [0002] Mycotoxin pollution has brought huge economic losses to the feed industry and animal husbandry, and seriously threatened the safety of human animal source food. The World Health Organization estimates that about 25% of the world's crops are contaminated by mycotoxins, and more than 2% are inedible, causing economic losses close to 1 trillion US dollars per year. The National Grain Administration found that the positive rate of mycotoxins in grains in my country is over 90%, and the post-harvest grain loss due to mildew is as high as 21 million tons per year (accounting for 4.2% of the total grain output), and the direct economic loss is about 18-24 billion yuan per year. , the indirect loss exceeds 100 billion yuan / year. [0003] Mycotoxins are toxic se...

AI Technical Summary

Problems solved by technology

At present, the research on the biodegradation of mycotoxins in China mainly focuses on the screening and detoxification ability of strains degrading a single toxin, but there is little research on the screening of strains with high activity and simultaneous degradation of multiple mycotoxins

Due to the particularity of the source of biological enzymes, specificity of action and harsh application conditions, there are few studies on mycotoxin-degrading enzymes and their application in actual production.

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