Tissue culture and rapid propagation method for variation malus spectabilis
A tissue culture, red fruit technology, applied in horticultural methods, botanical equipment and methods, horticulture and other directions, can solve the problems of insufficient grafting branches, seedling character separation, etc., and achieve the effects of fast reproduction, high rooting rate, and pollution reduction.
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[0022] The present invention will be further described below in conjunction with specific examples, but the examples do not limit the present invention in any form. Unless otherwise specified, the reagents, methods and equipment used in the present invention are conventional reagents, methods and equipment in the technical field. Unless otherwise specified, all reagents and materials used were commercially available.
[0023] This method was carried out in the tissue culture laboratory of Jiangsu University in Zhenjiang City, Jiangsu Province. Some improvements were made on the traditional tissue culture method of Malus, and the tissue culture propagation method of the variant winter red fruit was introduced with emphasis. The operation is simple and the survival rate is high, and the Tissue culture propagation efficiency of variant Donghong fruit. Variation winter red fruit tissue culture rapid propagation method implementation steps are as follows:
[0024] 1. Explant sele...
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