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Tissue culture and rapid propagation method for variation malus spectabilis

A tissue culture, red fruit technology, applied in horticultural methods, botanical equipment and methods, horticulture and other directions, can solve the problems of insufficient grafting branches, seedling character separation, etc., and achieve the effects of fast reproduction, high rooting rate, and pollution reduction.

Inactive Publication Date: 2019-04-02
JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the traditional grafting propagation method does not have enough grafted branches, and the seedlings obtained by sowing have obvious trait separation

Method used

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Examples

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Embodiment Construction

[0022] The present invention will be further described below in conjunction with specific examples, but the examples do not limit the present invention in any form. Unless otherwise specified, the reagents, methods and equipment used in the present invention are conventional reagents, methods and equipment in the technical field. Unless otherwise specified, all reagents and materials used were commercially available.

[0023] This method was carried out in the tissue culture laboratory of Jiangsu University in Zhenjiang City, Jiangsu Province. Some improvements were made on the traditional tissue culture method of Malus, and the tissue culture propagation method of the variant winter red fruit was introduced with emphasis. The operation is simple and the survival rate is high, and the Tissue culture propagation efficiency of variant Donghong fruit. Variation winter red fruit tissue culture rapid propagation method implementation steps are as follows:

[0024] 1. Explant sele...

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PUM

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Abstract

The invention discloses a tissue culture and rapid propagation method for variation malus spectabilis, and belongs to the field of plant tissue culture and propagation. The tissue culture and rapid propagation method has the advantages that processes for explant selection and disinfection, primary culture, secondary culture, rooting culture, transplanting and the like in variation malus spectabilis tissue culture are provided, accordingly, the contamination rate of the variation malus spectabilis tissue culture and the death rate of tissue culture seedlings due to improper handling can be reduced, and the variation malus spectabilis tissue culture efficiency can be improved.

Description

technical field [0001] The invention belongs to the field of plant tissue culture and breeding, and in particular relates to a tissue culture rapid breeding method of mutated winter red fruit. Background technique [0002] Donghongguo is a deciduous small tree of the Rosaceae apple genus. The leaves and veins are green, the flower color is light pink, it blooms in spring, and it bears fruit immediately. The fruit is clustered, and the fruit color is green at first, and then turns yellow. After late autumn, the leaves fall off. From February to March of the second year, it is also called longevity fruit, so it is of high ornamental value. At the same time, the plants are relatively short and the tree shape is compact, which is very suitable for viewing as bonsai, suitable for indoor and outdoor placement, and is deeply loved by the masses. Winter red fruit propagation generally uses apple seedlings as rootstock grafting, and it can bloom and bear fruit in the second year. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/005A01H4/008
Inventor 付为国韦晨薛珂
Owner JIANGSU UNIV
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