Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Application of RNA polymerase III transcribed AtR8 long noncoding RNA (lncRNA) to arabidopsis thaliana

An RNA polymerase, non-coding technology, applied in the direction of DNA / RNA fragments, applications, recombinant DNA technology, etc., can solve problems such as unclear molecular mechanisms, and achieve the effect of increasing sensitivity

Active Publication Date: 2019-03-29
NORTHEAST FORESTRY UNIVERSITY
View PDF1 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the detailed molecular mechanism of seed germination is still unclear

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of RNA polymerase III transcribed AtR8 long noncoding RNA (lncRNA) to arabidopsis thaliana
  • Application of RNA polymerase III transcribed AtR8 long noncoding RNA (lncRNA) to arabidopsis thaliana
  • Application of RNA polymerase III transcribed AtR8 long noncoding RNA (lncRNA) to arabidopsis thaliana

Examples

Experimental program
Comparison scheme
Effect test

specific Embodiment approach 1

[0035] Specific embodiment one: the application of the AtR8 long non-coding RNA transcribed by RNA polymerase III in Arabidopsis thaliana of the present embodiment, the long non-coding RNA participates in the growth and development of Arabidopsis seeds under ABA stress conditions; the described The concentration of ABA is 5-20 mmol / L; the AtR8 long non-coding RNA nucleotide sequence transcribed by RNA polymerase III is shown in Seq ID No: 1 in the sequence table.

specific Embodiment approach 2

[0036] Specific embodiment 2: The difference between this embodiment and specific embodiment 1 is that the expression level of the long non-coding RNA increases under ABA stress conditions to promote Arabidopsis seed germination, but its deletion inhibits Arabidopsis seed germination. Others are the same as in the first embodiment.

specific Embodiment approach 3

[0037] Specific embodiment three: this embodiment differs from specific embodiments one to two in that: the ABA stress condition is: Arabidopsis seeds are sown on 1 / 2MS solid medium containing a concentration of 5-20mmol / LABA. After imbibition treatment at 4°C for 24-72h, culture in an incubator at 22°C, 16h light and 8h dark.

[0038] Others are the same as one of the specific embodiments 1 to 2.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides an application of RNA polymerase III transcribed AtR8 long noncoding RNA (lncRNA) to arabidopsis thaliana, relates to an application of long noncoding RNA to the arabidopsis thaliana, and aims to solve the problem of an arabidopsis thaliana seed germinating physiology mechanism not clear in a molecular layer, and particularly solve the problem of the seed germinating mechanism with AtR8 long noncoding RNA. The situation that the AtR8 lncRNA is RNA polymerase III transcribed long noncoding RNA in response to ABA stress in an arabidopsis thaliana seed germinating course isfound. The situation that the AtR8 lncRNA participates in the arabidopsis thaliana seed germinating course under ABA stress is proved. Under the normal growth condition, the AtR8 lncRNA participatesin growth and development of siliques, rosette leaves and seeds of arabidopsis thaliana. Through analysis, the AtR8 lncRNA possibly plays an important role in normal development of the arabidopsis thaliana and seed germination under ABA stress through influencing the expression of ABA signal pathway key genes ABI5 and EM6. The RNA polymerase III transcribed AtR8 long noncoding RNA (lncRNA) in arabidopsis thaliana disclosed by the invention is applied to the field of the arabidopsis thaliana.

Description

technical field [0001] The present invention relates to the application of long non-coding RNA in Arabidopsis. Background technique [0002] Long noncoding RNAs (lncRNAs, long noncoding RNAs) are a class of noncoding RNAs longer than 200 nucleotides that do not encode proteins. They are mainly composed of introns, promoters, intergenic sequences, and antisense protein-coding genes. The sequence is generated by RNA polymerase II transcription. Mammalian studies have shown that long noncoding RNAs play an important role in biological processes such as dosage compensation, genomic imprinting, and X chromosome inactivation. [0003] Compared with mammals, the research on long non-coding RNAs in plants started late, especially the research results on function and mechanism are less. At present, some functional long non-coding RNAs transcribed by RNA polymerase II have been discovered, but the mechanism of action of only a few long non-coding RNAs has been clearly resolved. AtI...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N15/113C12N15/82A01H5/00A01H6/20
CPCC12N15/113C12N15/8267C12N15/8293C12N2310/10
Inventor 吴娟张楠刘自广刘春晓黄小庆彭疑芳张晓旭王昊杨贺
Owner NORTHEAST FORESTRY UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com