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Nucleic acid detection microfluidic chip based on modified capillaries and nucleic acid detection system

A microfluidic chip and detection system technology, applied in laboratory containers, enzymology/microbiology devices, specific-purpose bioreactors/fermenters, etc., can solve the problem of failing to realize nucleic acid automation and reduce the difficulty of integration and other problems, to achieve the effect of improving portability and flexibility, reducing operation steps, and simple operation steps

Active Publication Date: 2019-03-29
合肥中科易康达生物医学有限公司 +1
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

published in Lab on a Chip by Hui Junhou et al. proposed a polychitosan-based modified glass The capillary array nucleic acid in situ amplification method, which uses filter paper to absorb nucleic acid, realizes nucleic acid amplification without elution steps, which greatly reduces the difficulty of integration. "Extraction-amplification-detection" automated process

Method used

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  • Nucleic acid detection microfluidic chip based on modified capillaries and nucleic acid detection system
  • Nucleic acid detection microfluidic chip based on modified capillaries and nucleic acid detection system
  • Nucleic acid detection microfluidic chip based on modified capillaries and nucleic acid detection system

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specific Embodiment approach 2

[0063] Embodiment 2. This embodiment is the application process of the modified capillary-based nucleic acid detection microfluidic chip and nucleic acid detection system described in Embodiment 1:

[0064] Step 1: Sample loading. The bottom of the microfluidic chip I is covered with thermal conductive silicone grease, and it is closely attached to the temperature control system VII. Place 250 μl of the blood sample to be tested and 500 μl of lysate in the first liquid storage chamber 61 and the second liquid storage chamber 62 respectively, and add 150 μl of PCRmix into the third liquid storage chamber 63; It is connected to each elastic film microvalve or syringe pump interface of the microfluidic chip 1 through a sealed hose.

[0065] Step 2: The sample is mixed with the lysate. Manipulate the mobile phone to control the control system IV to issue commands to start the air source 11, the solenoid valve and the injection pump. By opening the first solenoid valve 101 and t...

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Abstract

The invention relates to a nucleic acid detection microfluidic chip based on modified capillaries and a nucleic acid detection system. The microfluidic chip internally comprises modified capillaries which are used for adsorbing target nucleic acid in a sample to be tested, and are used as a carrier for realizing the automated implementation of nucleic acid adsorption, purification, amplification and detection. The invention realizes the functions of integrated sample lysis, nucleic acid extraction, nucleic acid amplification and nucleic acid detection, so that the chip can be deployed in non-laboratory areas such as entry and exit ports and open country, and detects infectious pathogens.

Description

technical field [0001] The invention relates to the technical field of microfluidic nucleic acid analysis chips, in particular to a nucleic acid detection microfluidic chip and a nucleic acid detection system based on a modified capillary. Background technique [0002] In recent years, several large-scale infectious diseases have broken out around the world, such as H1N1, Ebola virus, and Zika, etc., which require professional nucleic acid detection equipment to be detected through the process of nucleic acid extraction and amplification. Generally speaking, such instruments have the disadvantages of being bulky, difficult to deploy on-site, expensive and require dedicated maintenance. Microfluidic chips are widely used in the field of biochemical analysis, such as bacteria, virus and protein detection, due to their characteristics of easy integration, automation, fluid control and small sample size, which to a certain extent make up for professional nucleic acid detection e...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/00C12M1/38C12M1/34
CPCB01L3/5027B01L7/52B01L2200/027
Inventor 朱灵杨柯朱欣潮朱灿灿赵俊周喃汪磊邓国庆刘勇
Owner 合肥中科易康达生物医学有限公司
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