Method for preparing arginine-rich high-protein organic fertilizer by two-step fermentation process through using composite strain

Abstract

The invention discloses a method for preparing an arginine-rich high-protein organic fertilizer by a two-step fermentation process through using a composite strain. The method comprises the followingsteps: (1) respectively crushing plant dregs and plant meal, and uniformly mixing the crushed plant dregs and the crushed plant meal in proportion to prepare a mixture; (2) adding a molasses fermentation waste liquid to the mixture prepared in step (1) to prepare a fermentation medium; (3) inoculating the fermentation medium prepared in step (2) with a mixed strain A, and performing mixing fermentation at 28-32 DEG C for 24-28 h to obtain a pre-fermentation solution 1; (4) inoculating the pre-fermentation solution 1 with a mixed strain B, and carrying out mixing fermentation at 28-32 DEG C for24-48 h to obtain a post-fermentation solution 2; and (5) drying and crushing the post-fermentation solution 2 to obtain the arginine-rich high-protein organic fertilizer. The method has the advantages of safe process, convenience in preparation, and suitableness for large-scale industrial production.

Description

technical field [0001] The invention relates to the technical field of organic fertilizers, in particular to a method for preparing high-protein organic fertilizers rich in arginine by mixing strains and two-step fermentation in a culture medium. Background technique [0002] The annual output of yeast in the world is more than 3 million tons. The production of yeast is mainly based on sugarcane molasses and beet molasses. Furfural, furfural can be degraded by acclimatized anaerobic sludge, but the degradation performance of dark caramel compounds is very poor. Caramel compounds exist in yeast wastewater, which makes the color of yeast wastewater darker. At the same time, melanin, phenols, caramel, macromolecular organic matter, inorganic salts and SO in wastewater 4 2- The concentration is relatively high, and the wastewater is rich in inorganic trace elements such as nitrogen, phosphorus, potassium, calcium, iron, and organic substances such as humic acid and fulvic acid...

AI Technical Summary

Problems solved by technology

The physical method is mainly to remove anti-nutritional factors by heating, trypsin, etc. are sensitive to heat, but other inhibitory factors cannot be removed by heating, and heating will also destroy other nutrients; chemical methods are often not used, because chemical methods treat There will be a lot of chemical substances remaining in the process, resulting in difficulty in removal and increased cost. The biological method uses microorganisms to ferment defatted soybean meal, but the beany smell of the soybean meal fermentation liquid obtained by this method has not been well resolved

Peanut meal is currently mainly used for feed, and the added value of the product is low, resulting in a serious waste of resources