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Application of an extreme acid and alkali hydrolysis method in the production of iturin-a from Bacillus fermented oilseed meal and its method

A technology of iturin and bacillus, applied in the direction of microorganism-based methods, biochemical equipment and methods, fermentation, etc., can solve the problems of restricting industrial production and commercial application, difficulty in controlling fermentation process, and high cost, and achieve It is beneficial to industrialization and application, reduces fermentation cost and time, and saves energy

Active Publication Date: 2021-10-15
OIL CROPS RES INST CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, so far, there is still no feasible Iturin A fermentation production method at home and abroad. Its output is low, its cost is high, and its fermentation process is difficult to control, which restricts its industrial production and commercial application.

Method used

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  • Application of an extreme acid and alkali hydrolysis method in the production of iturin-a from Bacillus fermented oilseed meal and its method

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Embodiment

[0022] An application of extreme acid and alkali hydrolysis in the production of iturin A from bacillus fermented rapeseed meal, the application method is as follows: Rapeseed meal (90g / L), glucose (80g / L) and water are carried out in proportion Mix it as a medium, add an appropriate amount of hydrochloric acid or sodium hydroxide to the medium to control the concentration of hydrochloric acid or sodium hydroxide in the medium at 0.1-0.4mol / L, make the medium in an extreme acid and alkali environment, and then in 55 ℃, 150rpm / min under the conditions of hydrolysis for 2 hours; then use sodium hydroxide or hydrochloric acid to adjust the pH to 7; insert Bacillus amyloliquefaciens CX-20 according to the inoculation amount of 5%, ferment for 72 hours, and measure the production of Iturin A content, such as figure 1 shown.

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Abstract

The invention discloses the application of an extreme acid and alkali hydrolysis method in the production of iturin A from bacillus fermented oilseed meal, belonging to the field of microbial fermentation. The method comprises: adding an appropriate amount of hydrochloric acid or sodium hydroxide to a culture medium containing oilseed meal, carbon source and water, then hydrolyzing to achieve sterilization, then using alkali or acid to adjust the pH of the culture medium to 7, and then inserting spores Bacillus is fermented, so that the amount of Iturin A produced by Bacillus fermentation can reach a higher level.

Description

technical field [0001] The invention relates to the field of microbial fermentation, in particular to the application of an extreme acid and alkali hydrolysis method in the production of iturin A from bacillus fermented oilseed meal and the method thereof. Background technique [0002] Iturin A (Iturin A) is one of the lipopeptides synthesized by Bacillus. It has the characteristics of inhibiting cancer, hemolysis, surface activity and strong antifungal properties, and has the characteristics of broad antibacterial spectrum, low toxicity and small allergic reaction. An antifungal preparation with great application potential can be widely used to prevent and control fungal diseases of various crops, vegetables and fruits. A new type of biopesticide with great research and development value and application prospect has attracted widespread attention at home and abroad in recent years. However, so far, there is still no feasible Iturin A fermentation production method at home ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P21/04C12R1/07
CPCC07K7/56C12P21/02
Inventor 陈文超万霞李旋马旭丽黄凤洪郑明明
Owner OIL CROPS RES INST CHINESE ACAD OF AGRI SCI
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