Bacillus amyloliquefaciens GUAL210 and application thereof
A technology of amylolytic spores and bacilli, applied in the direction of application, bacteria, fungicides, etc.
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[0017] Below in conjunction with accompanying drawing and specific embodiment the invention is further introduced:
[0018] 1. Isolation and screening of Bacillus amyloliquefaciens GUAL210
[0019] 1. Materials and methods
[0020] 1.1 Materials
[0021] Soil samples were collected from the plots of tobacco black shank and bacterial wilt in Anlong County, Qianxinan Prefecture, Guizhou Province from 2017 to 2018. The 5-point sampling method was used to collect soil samples from the same plot. Mixing counts as 1 soil sample. Use paper bags to collect soil samples, and disinfect soil collection tools in advance to avoid mixing soil microorganisms.
[0022] 1.2 Medium
[0023] LB medium is used for the isolation and preservation of Bacillus and plate confrontation experiments; PDA medium is mainly used for plate confrontation experiments.
[0024] 1.3 Tobacco disease strains
[0025] The tested strains of tobacco black shank (Phytophthora nicotianae) and tobacco bacterial wi...
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