Kit for detecting human immune ages and application of kit
A technology for detecting human body and kit, applied in the field of medicine, can solve the problem of lack of a mature detection method, and achieve the effect of good repeatability, high sensitivity and strong specificity
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preparation example Construction
[0068] The preparation method of the kit comprises the following steps:
[0069] 1) Fluorescently label the antibody;
[0070] 2) Metering the fluorescently labeled antibody according to the formula amount;
[0071] 3) Add the fluorescently labeled antibody measured above into a light-proof plastic or glass reagent bottle, mix evenly, and screw the cap to seal to obtain a kit.
[0072] The storage conditions of the kit are: 2-8°C, protected from light.
[0073] After the preparation of the kit is completed, the human peripheral blood from the above 800 samples is tested; the human body includes physical examination persons of all ages, and is required to be free of thymoma or thymus-related diseases, and is not used to stimulate the immune system. drug.
[0074] When the kit is used for detection, the preparation of the detection sample includes the following steps:
[0075] 1) Collect not less than 100 μL of human peripheral blood as a test sample; the test sample is test...
Embodiment 1
[0089] Preparation of reagents:
[0090] CD3-FITC, CD4-PerCP, CD31-PE, CD45RA-APC four kinds of monoclonal antibodies, take 125 μ L of each antibody respectively, add in light-proof plastic or glass reagent bottle, mix evenly, namely become 100 servings of the present invention product, corresponding to the dosage of the product per person is 5 μL.
Embodiment 2
[0092] Preparation of reagents:
[0093] CD3-FITC, CD4-PerCP, CD31-PE, CD45RA-APC four kinds of monoclonal antibodies, take 50 μL of each antibody respectively, add in light-proof plastic or glass reagent bottle, mix evenly, namely become 100 servings of the present invention product, corresponding to the dosage of the product per person is 2 μL.
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