MiRNA detection kit and application thereof in diagnosis of degree of coronary stenosis

Abstract

The invention provides a complete detection kit for conveniently and rapidly detecting miRNA. According to the detection kit, a special primer sequence does not need to be customized. On the other hand, the detecting kit is used for detecting the expression level of circulating miR-155 in serum of an AS patient and the expression level of miR-155 in a plaque tissue and simultaneously detecting theexpression levels of miR-155 in the serum of the patients with different degrees of coronary stenosis, the correlation between miR-155 and AS common risk factors is analyzed by virtue of a statistical method, the diagnosibility of miR-155 is analyzed by virtue of ROC when the degree of coronary stenosis is more than 75%, the expression of miR-155 in AS and the clinical significance are discussed,and a new through is provided for the auxiliary diagnosis of AS and the grading of the degree of coronary stenosis.

Description

technical field [0001] The invention belongs to the technical field of molecular biology, and in particular relates to a detection kit of miRNA and its application as a molecular marker in diagnosing the stenosis degree of coronary artery lumen. Background technique [0002] The pathogenesis of atherosclerosis (AS) is a complex disease with polygenetic inheritance, multi-level involvement of multiple immune and vascular wall cell components, and multi-stage long-term development, which can cause different degrees of vascular lumen Stenosis leads to clinically serious events such as blockage, most commonly in the arterial system involving the coronary arteries. Further development of coronary atherosclerosis can narrow the lumen, reduce blood flow, and necrosis of the myocardium due to ischemia, leading to serious diseases such as angina pectoris, acute myocardial infarction, ventricular arrhythmia, and sudden death; in the past ten years of statistics , coronary atheroscler...

AI Technical Summary

Problems solved by technology

[0007] At present, the detection methods of miRNA research at home and abroad are mainly chip technology, sequencing technology and real-time fluorescent quantitative PCR reaction. Chip technology can carry out large-scale detection and screening, but due to high cost and strict requirements for detection samples, chip technology is not used as a routine The method of detecting miRNA; sequencing technology can accurately confirm the type of miRNA by detecting the base type, but it is also expensive, time-consuming and cannot be accurately quantified, so it is not adopted as a routine method for miRNA detection; real-time fluorescence quantification PCR technology has the advantages of simple and convenient operation, relatively quantitative miRNA content, and low cost, making it a routine method for miRNA detection. However, most of the PCR kits for miRNA detection on the market require additional It is inconvenient to formulate special miRNA reverse transcription primers and use upstream and downstream primers for quantitative PCR detection

[0008] The severity of atherosclerosis can be judged by detecting the level of miRNA expression. Due to the many pathogenic factors of atherosclerosis, the diagnostic results of patients with coronary stenosis often have large errors or misjudgments. Low

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