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Substrate impregnated with at least one composition for the sampling of microorganisms present on a surface

A technology of microorganisms and compositions, which is applied in the determination/inspection of microorganisms, detergent compositions, aqueous liquid soap-free compositions, etc., and can solve the problems of ineffective collection of microorganisms

Inactive Publication Date: 2019-03-15
雷亚尔科公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0020] Unfortunately, microbes that have formed biofilms do not appear to be efficiently collected using this sampling device

Method used

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  • Substrate impregnated with at least one composition for the sampling of microorganisms present on a surface
  • Substrate impregnated with at least one composition for the sampling of microorganisms present on a surface
  • Substrate impregnated with at least one composition for the sampling of microorganisms present on a surface

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0077] Example 1: Effect of sampling from surfaces on microorganisms protected by biofilms, depending on whether the substrate according to the invention is impregnated with compositions of different dynamic viscosities

[0078] Impregnation of different dynamic viscosities of the matrix according to the invention for sampling microorganisms present on a surface, in particular microorganisms forming a biofilm on the surface, has been tested in terms of their effect on sampling microorganisms from the surface. For this, the following process is observed.

[0079] a) Process of developing biofilm on stainless steel surface

[0080] Day -1: Erlenmeyer flasks containing 20 ml sterile TSB medium (Tryptic Soy Broth) were inoculated with S. epidermidis culture while maintaining stirring (120 rpm) at 35°C overnight.

[0081] Day 0:

[0082] - Sterile stainless steel surfaces were placed in sterile Petri dishes with hydrophobic lines drawn along the surface contours to keep BSA conta...

Embodiment 2

[0108] Example 2: Adhesion of the substrate on vertical and horizontal surfaces depending on the dynamic viscosity of the composition impregnated in the sampling substrate according to the invention

[0109] The sampling procedure as described in Example 1 applied three solutions (A, B, C) of different viscosities. For each solution (composition), testing was performed on both vertical and horizontal surfaces, as being able to collect below the surface could become of particular interest in the industry.

[0110] Several parameters were determined after application of the sampling matrix:

[0111] - Cloth penetration and its composition and distribution;

[0112] - hold the cloth on the surface;

[0113] - Runoff of the sampled composition from the cloth to the surface; and

[0114] - Distribution of the sampling composition on the surface when the cloth is removed from the surface: when the first cloth is removed, the surface should preferably be completely covered with a ...

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Abstract

The invention relates to a substrate impregnated with at least one composition comprising at least one enzymatic component for the sampling of microorganisms present on a surface, in particular for the sampling of microorganisms that may or may not have formed a biofilm on said surface, said at least one composition having a dynamic viscosity of between 50 and 2000 mPa.s.

Description

technical field [0001] The present invention relates to a substrate impregnated with at least one composition comprising at least one enzyme component, said substrate being used for sampling microorganisms present on a surface, in particular for the detection of microorganisms formed on said surface or microorganisms that do not form biofilms. [0002] According to the invention, the term "impregnated substrate" means a substrate in which the liquid is dispersed or diffused in all parts. The term infiltrated matrix may also be used, the terms "impregnated" and "infiltrated" being used interchangeably according to the present invention. Background technique [0003] In many areas of work such as the agri-food sector, rural communities, medical and veterinary sectors, problematic contamination due to the presence of microorganisms is often observed. Recently, however, stringent hygiene standards are required and it is necessary to ensure that the number of microorganisms cau...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C11D3/386C11D3/48C11D17/04
CPCC11D3/386C11D3/48C11D3/046C11D3/222C11D3/3765C11D17/003C12Q1/025C12Q1/06C12Q1/18
Inventor 戈登·布莱克曼塞巴斯汀·法斯特兹卡米尔·盖斯
Owner 雷亚尔科公司
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