Genetic molecular marker co-segregated with male sterility gene msc-2 of capsicum annuum cell nucleus and application of genetic molecular marker

Abstract

The invention discloses a genetic molecular marker co-segregated with a male sterility gene msc-2 of a capsicum annuum cell nucleus and an application of the genetic molecular marker. Primers for detecting capsicum annuum fertility are provided and are a primer pair capable of amplifying a specific fragment; the specific fragment comprises a target sequence of the primer pair consisting of a primer F and a primer R; the primer F is following a1) or a2): a1) single stranded DNA molecules represented as a sequence 1 of a sequence table; a2) DNA molecules which are obtained by substitution and / ordeletion and / or addition of the sequence 1 by one or more nucleotides and have the same function with the sequence 1; the primer R is following a3) or a4): a3), single stranded DNA molecules represented as a sequence 2 of the sequence table; a2) DNA molecules which are obtained by substitution and / or deletion and / or addition of the sequence 2 by one or more nucleotides and have the same functionwith the sequence 2. Fertile plants can be rapidly identified in the seedling stage according to the developed marker.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a gene molecular marker co-separated with the pepper nuclear male sterility gene msc-2 and an application thereof. Background technique [0002] Pepper (Capsicum annuum L.) is a herbaceous plant belonging to the family Solanaceae (Solanaceae), also known as pepper, sea pepper, spicy eggplant, pepper, etc. According to statistics, the global planting area of ​​pepper has exceeded 50 million mu, with an annual output of 37 million tons. It is the largest seasoning crop in the world. my country's pepper planting area exceeds 24 million mu, accounting for more than 35% of the world's pepper area and about 10% of the country's vegetable area, second only to the cabbage planting area. The total economic output value of pepper exceeds 70 billion yuan, ranking first in terms of output value and benefits. The first vegetable crop (China Vegetable Circulation Association, 2017). P...

AI Technical Summary

Problems solved by technology

Except for a gene marker developed by msc-1, the rest of the molecular markers linked to the ms gene either have a long linkage distance or high cost of marker use, and their applicability needs to be improved

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